磺胺母核抗原合成及多克隆抗体的制备  

Synthesis of Artificial Antigens for Sulfanilamides and the Preparation of Its Antiserum

作　　者：郭玮[1] 裴兆柱[1,2] 王星星[1] 吴国娟[1] 

机构地区：[1]北京农学院动物科学技术系,北京102206 [2]北京市崇文区动物卫生监督所,北京100051

出　　处：《北京农学院学报》2008年第4期33-36,共4页Journal of Beijing University of Agriculture

基　　金：北京市农委科技攻关资助项目(项目编号:20020103);国家自然科学基金项目(项目编号:30771625)

摘　　要：制备磺胺类药物母核结构的半抗原(H),通过免疫家兔得到多克隆抗体,为制备针对多种磺胺类药物的单抗和磺胺药检测免疫胶体金试纸条研制奠定基础。以对乙酰氨基苯磺酰氯(ASC)和对氨基苯甲酸甲酯(PA-BA)合成磺胺母核的半抗原(H);以牛血清白蛋白(BSA)、卵清蛋白(OVA)为载体,采用混合酸酐法,合成了免疫原H-BSA和包被原H-OVA通过紫外光谱定性证明偶联物偶联成功与否,以H-BSA免疫家兔,制备多克隆抗体,ELISA法对其特异性及效价进行检测。成功合成了磺胺母核人工抗原即免疫原H-BSA和包被原H-OVA,二者的蛋白浓度分别为8.46 mg/mL和10.53 mg/mL,结合比分别为13∶1和9∶1;制备的多克隆抗体特异性良好,血清效价为1∶256 000。To lay a foundation for the preparation of sulfanilamides＇ monoclonal antibody and the development of its gold-immunochromatographic assay, Hapten H was synthesized and its antiserum were prepared. Nacetylsulianily chloride and para-amino benzoic acid were used to synthesize Hapten （H）, and it was coupled to carrier protein bovine serum albumin（BSA） and ovalbumin（OVA） by using the mixed acid ahhdride method. The conjugates were identified by UV absorption method to calculate the coupling ratio and the protein concentration. H-BSA was used as immunogen to inject the rabbits to prepare antiserum. The affinity and the titer of the antiserum were detected by using the H-OVA as coating antigen. Sulfanilamides＇ artificial antigens were synthesized successfully. The protein concentration of H-BSA was 8.46 mg/mL or 10.53 mg/mL for H-OVA, respectividy. The coupling ratio of H to BSA was 13 ： 1 and to OVA 9 ： 1. The titer of antiserum was 1 ： 256 000.

关 键 词：磺胺类药物 人工抗原 多克隆抗体 

分 类 号：S852.4[农业科学—基础兽医学]