流动注射化学发光法测定人体血清和金银花中的绿原酸  

Determination of chlorogenic acid using chemiluminescence with flow injection analysis in human serum and Flos Lonicerae

作　　者：邱小香[1] 宋正华[2] 

机构地区：[1]渭南师范学院化学化工系,陕西渭南714000 [2]西北大学化学系,陕西西安710069

出　　处：《纺织高校基础科学学报》2008年第4期497-500,共4页Basic Sciences Journal of Textile Universities

基　　金：渭南师院自然科学基金资助项目(07YKZ005)

摘　　要：基于绿原酸对鲁米诺-溶解氧体系化学发光反应的抑制作用，结合流动注射技术，建立了绿原酸化学发光分析法，用于人体血清、金银花水提液中绿原酸测定，实现了样品中超痕量的分析．当绿原酸的质量浓度为1．0ng·mL^-1～100．0ng·mL^-1时，化学发光强度的减小值与其浓度呈线性关系．测定的检出限为0．33ng·mL^-1，线性相关系数r^2=0．9978，加标回收率为95．8％～105．5％．在流速为2．0mL·min^-1时，该方法完成一次绿原酸的测定只需0．5min，相对标准偏差为5．0％，并且选择性和灵敏度较高．Based on the inhibitive effect of chlorogenic acid on the chemiluminescence reaction between luminol and dissolved oxygen in a flow injection systen,a sensitive chemiluminescence was proposed for the determination of ultra-trace chlorogenic acid. The decrease of CL intensity correlates linearly with cholorogenic acid concentration in the range from 1.0 ng · mL^-1 to 100.0ng · mL^-1 （r^2 =0. 997 8）,and the detection limit is 0. 33ng · mL^-1, At a flow rate of 2.0mL · min^-1, a complete analytical process could be performed in 0. 5min, a relative standard deviationis is less than 5%. The sensitive flow infection method was applied successfully to determine the chlorogenic acid in Flos Lonieerae, human serum without any pretreatment procedure,with recovery from 95.8% to 105.5%.

关 键 词：流动注射 化学发光 绿原酸 人体血清 金银花 

分 类 号：O657.39[理学—分析化学]