Wistar大鼠血液生化指标正常参考值范围的研究  

Research of Normal Reference Range of Blood Biochemical Indicators of Wistar Rats

作　　者：郭建生[1] 胡还甫[1] 郭璇[1] 

机构地区：[1]湖南中医药大学,湖南长沙410007

出　　处：《中医药导报》2008年第12期7-8,共2页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：湖南省中医药管理局资助项目(204085)

摘　　要：目的:制定Wistar大鼠血液生化指标正常值。方法:在严格控制条件的情况下,从长毒实验中的空白对照组筛选10批Wistar大鼠(初始体重60-90g,每批30只),雌雄各150只。连续饲养观察12周后,雌性大鼠体重为(241.29±19.62)g,雄性为(347.57±25.73)g。根据血液生化指标检测结果,通过统计分析,研究确定其正常参考值范围。结果:结果显示Wistar大鼠血液生化指标中,谷丙转氨酶、总蛋白、白蛋白、尿素氮、肌酐和胆固醇6项指标雄雌差异具有统计学意义,谷草转氨酶、血糖、球蛋白、碱性磷酸酶指标雄雌差异无统计学意义。结论:研究和确定Wistar大鼠血液生化指标的正常值范围,可以为药物的安全性评价以及国家标准和规范的修订提供参考依据。Objective： To make normal reference of blood biochemical indicators of Wistar rats. Methods： In strictly controlled conditions, to select 10 approved Wistar rats （initial weight 60-90 g, each 30） from the control group with the experiment of long drugs, male and female each 150.After continuous observation of keepingl2 weeks, the female rats weighed （241.29±19.62） g, male for （347.57±25.73） g. According to test results of blood biochemical indicators, through statistical analysis, to study and identify its normal reference range. Results： The result showed that among the biochemical indicators of Wistar rats ,there was a significant difference between male and female rats on alanine aminotransferase（ALT）,total protein（TPRO）, albumin（ALB）, blood urea nitrogen（BUN）, creatinine（Cr）, and cholesterol（TCHO）;while there was no significant difference betweenmale and female rats on aspartate aminotransferase（AST）, glucose（GLU）, globulin（GLB）, alkalinephosphatase （ALP）. Conclusion：to research and identify the normal reference range of blood biochemical indicators of Wistar rats, can provide reference basis for the safety of drug evaluation, as well as the amendment of national standards and norms.

关 键 词：WISTAR大鼠 生化指标 正常值 

分 类 号：Q461[生物学—生理学]