大鼠冠状动脉微栓塞模型的建立  

The Establishment of A Model of Rat Coronary Microembolization

作　　者：朱汉华[1] 李浪[1] 汪熠[1] 陆永光[1] 赵献明[1] 文伟明[1] 

机构地区：[1]广西医科大学第一附属医院心内科广西心血管病研究所,广西南宁530021

出　　处：《中国微循环》2008年第6期380-382,384,404,共5页Journal of Chinese Microcirculation

基　　金：国家自然科学基金资助项目(C030313);广西研究生教育创新计划资助项目(2006105981002M08)

摘　　要：目的建立大鼠冠状动脉微栓塞(Coronary Microembolization,CME)模型。方法S-D雄性大鼠随机分为假手术组(S0组),微栓塞组(CME组);CME组再按微球数目不同分为1000、2000、3000、4000个微球亚组(分别计为CME1、CME2、CME3、CME4组,各组存活大鼠均n=10);大鼠麻醉后开胸,夹闭升主动脉10s,从左心室注射微栓塞球到达冠状动脉记为CME组,以注射生理盐水为假手术组;分别于术后6h心脏超声检测左室射血分数(LVEF)、HBFP检测心肌微梗死面积和TUNEL检测心肌细胞凋亡率。结果与S0组比较,CME1组LVEF下降,但没有统计学意义;与S0组比较,CME2、CME3、CME4组LVEF均显著下降(均P<0.05);CME组均出现心肌微梗死灶与心肌细胞凋亡。②不同微栓塞亚组之间比较,LVEF与微栓塞球数目成负相关(γ=-0.78,P<0.05)、心肌微梗死面积和心肌细胞凋亡率均与微栓塞数目成正相关(γ分别为0.85、0.80,均P<0.05)。③3000个微球是较理想的建立大鼠CME模型所需的微球数目。结论开胸大鼠,从左室注入微栓塞球3000个,可成功建立大鼠CME模型。Objective To produce coronary microembolization model in rats with microspheres. Methods Coronary embolization models were produced by injection of 42μm microspheres（1000, 2000, 3000, 4000/0.1 ml, respectively） into the left ventricle during 10 seconds ascending aorta occlusion in adult male Sprague-Dawley rats（ CME group）. The survivors were randomly divided into CME1, CME2, CME3, CME4 subgroups （ n = 10, respectively）. The sham group was injected saline（0.1 ml） instead（S0 group, n = 10 ）. Left ventricular ejection fraction（LVEF） was assessed by transthoracic two-dimensional echocardiography. Microinfarction size was detected with hematoxylin basic fuchsin picric acid （HBFP） staining. Cardiomyocyte apoptosis was detected by using TUNEL staining. Results ①Compared with SO group, LVEF was significally decreased in CME groups except CMEI subgroup（P〈0.05）. Microinfarction and cardiomyocyte apoptosis can be detected in CME groups.②LVEF was negatively correlative with the numbers of microspheres （γ= -0.78, P 〈 0.05）. Microinfarction size was positively correlated with the numbers of microspheres（γ= 0.85,P〈0.05）. Cardiomyocyte apoptosis rate was positively correlated with the numbers of microspheres（γ= 0.80,P〈0.05）. ③3000 is the suitable number of microspheres in this rat coronary microembolizatiou model. Conclusion Coronary microembolization model was successfully induced after injecting microspheres（ 3000/0.1 ml） into the coronary artery. The model is useful for the investigation of the patho- physiology of coronary microcirculation.

关 键 词：冠状动脉微栓塞 微球 大鼠 动物模型 微梗死 

分 类 号：R541.4[医药卫生—心血管疾病]