万寿菊种子带菌检测及种子消毒处理研究  

Testing of seed borne fungi and seed disinfection of Tagetes erecta

作　　者：何冬云[1] 王龙[1] 张肖凌 王生荣[1] 

机构地区：[1]甘肃农业大学草业学院植物病理系,甘肃兰州730070 [2]甘肃农垦农业研究院,甘肃武威733006

出　　处：《甘肃农业大学学报》2008年第6期99-101,共3页Journal of Gansu Agricultural University

基　　金：甘肃农垦研究院资助研究(GS-036179).

摘　　要：采用PDA平板分离培养检验法对5个品种的万寿菊种子表面进行带菌检测，并测定5种杀菌剂和2种温汤浸种方法对种子的消毒处理效果．结果表明。万寿菊种子表面带菌率在6．0％～85．0％之间，携带真菌的优势菌群主要是链格孢属真菌（Alternaria spp．），其分离频率最高达60．16％；其次为镰刀菌属（Fusarium spp．），分离频率为20．76％；青霉属（Penicillium spp．）分离频率为8．76％，黑根霉属（Rhizopusspp．）分离频率为3．08％、曲霉属（Aspergillus spp．）分离频率为2．08％。毛霉属（Mucor spp．）分离频率为1．40％，同时还分离到细菌．分离频率为6．92％，同一品种各菌群分离频率差异显著；用0．1％AgNO3和55℃温汤浸种可以杀死种子表面所有真菌，50℃温汤浸种和50％扑海因WP750倍液的消毒效果也明显高于其它处理，是较理想的种子消毒方法．A test petri-dish testing, and of seed borne fungi on 5 cultivars of Tagetes erecta were carried out by the method of the disinfection effects of 5 fungicides and two hot water dips were determined. The results showed that the seed borne fungi rate of Tagetes erecta ranged from 6.0 % to 85.0 %. The dominant microflora of the seed borne fungi was Alternaria spp. ,and its isolation frequency was 60.16 % ,then the Fusarium spp. ,its isolation frequency was 20.76 % ,and the isolation frequency of other fungi such as Penicillium spp. ,Rhizopus spp. ,Aspergillus spp. and Mucor spp. were as 8.76%, 3.08 %,2. 08 % and 1.4 % respectively. Both treatments with 0.1 % AgNO3 and 55℃ hot water dip could kill all kinds of fungi on seed surface completely. The disinfection effects of 50℃ hot water dip and 50% iprodione WP（750）were obviously better than other ways, and could be used as ideal disinfection method.

关 键 词：万寿菊 种子带菌 种子消毒处理 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治] S432.9[农业科学—植物保护]