水稻品种宁317对水稻条纹叶枯病的抗性特征及其遗传  

Mechanism and Inheritance of Resistance to Rice Stripe Disease in a Rice Variety Ning 317

作　　者：周彤[1] 王磊[1] 程兆榜[1] 范永坚[1] 周益军[1] 汤陵华[2] 方先文[2] 

机构地区：[1]江苏省农业科学院植物保护研究所,江苏南京210014 [2]江苏省农业科学院粮食作物研究所,江苏南京210014

出　　处：《江苏农业学报》2008年第6期756-761,共6页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省农业科技自主创新基金项目[CX(08)129、CX(08)603];国家支撑计划项目(2006BAD02A16、2006BAD08A04、2006BAD13B01-17);江苏省高技术项目(BG2006301);农业部行业专项(nyhyzx07-051);江苏省“333”工程人才项目

摘　　要：为了明确水稻品种宁317对水稻条纹叶枯病的抗性特征及其抗性遗传模式,并寻找与抗条纹叶枯病基因连锁的分子标记。利用单分蘖鉴定、苗期接种鉴定、非嗜性测验和抗生性测验分析宁317对水稻条纹叶枯病和介体灰飞虱的抗性;并分别采用单分蘖鉴定和苗期接种鉴定方法对武育粳3号与宁317构建的F2群体、F2∶3家系进行水稻条纹叶枯病抗性遗传分析;同时以SSR标记连锁分析方法对抗病基因进行定位。结果表明:宁317表现出较强的病毒抗性和较弱的抗虫性;其对水稻条纹叶枯病的抗性表现为显性单基因的遗传模式;SSR标记分析结果显示该抗病基因位于水稻第11染色体上,与标记RM287间的遗传距离为21.7 cM。这表明宁317对水稻条纹叶枯病的抗性主要来自于对病毒的抗性,由1对主效核基因控制,以宁317为抗病亲本结合本研究结果,可望加快水稻条纹叶枯病抗性品种选育的速度。The objective was to analyze the resistance mechanism and resistance genetic model to rice stripe disease in Ning 317 ,and to screen the SSR marker related to the gene of rice stripe disease resistance in Ning 317. The resistance to rice stripe virus （RSV） and the vector, small brown planthopper （SBPH） （Laodelphax striatellus Fallèn）was analyzed in Ning 317 by using the method of tiller inoculation,inoculation at the seeding stage, non-preference test and antibiosis test. The inheritance of rice stripe disease resistance in F2 lines and F2：3 lines from the cross Ning 317×Wuyujing No. 3 were studied by using tiller inoculation and inoculation at the seeding stage,respectively. The resistance gene was mapped based on SSR analysis. The results showed that Ning 317 was highly resistant to rice stripe virus and weakly resistant to vector. Genetic analysis indicated that the resistance to rice stripe disease in Ning 317 was inherited as a single dominant trait. The rice stripe disease resistance gene in Ning 317 was located on the chromosome 11 ,with the distance of 21.7 cM from a SSR marker RM287. Therefore, the rice stripe disease resistance in Ning 317 was mainly derived from the resistance to virus and controlled by a single major gene. The breeding process for rice stripe disease resistance should be accelerated by using Ning 317 as a resistance parent.

关 键 词：水稻条纹叶枯病 水稻品种 抗性特征 遗传分析 基因定位 

分 类 号：Q343.17[生物学—遗传学]