水貂病毒性肠炎巢式PCR,PCR-RFLP联合诊断方法的建立  

Development of A Nested PCR and PCR-RFLP for Detection of Mink Enteritis Virus

作　　者：易立[1] 闫喜军[1] 罗彬[1] 王建科[1] 赵传芳 吴威 程世鹏[1] 

机构地区：[1]中国农业科学院特产研究所,吉林132109 [2]吉林中特生物技术有限责任公司,吉林132109

出　　处：《经济动物学报》2008年第4期204-208,共5页Journal of Economic Animal

基　　金：吉林省科技发展计划项目(20080213)

摘　　要：根据水貂肠炎细小病毒的VP2基因序列设计4条特异性引物,建立了检测水貂肠炎细小病毒的巢式PCR方法。采用一次扩增的敏感性来检测5个TCID50/mL的细小病毒MEVB株,二次扩增的敏感性来检测0.05个TCID50/mL的细小病毒MEVB株。同时,利用该方法第一轮PCR产物酶切,能够区分犬细小病毒与水貂肠炎细小病毒,具有重要的实用价值和应用的前景。According to the published VP2 gene of mink enteritis virus, we designed four specific primers named MI, M2, M3 and M4 and set up a nested PCR assay to detect mink enteritis virus. Sensitive assay showed that the nested PCR could detect 5.0 TCID50 and 0.05 TCID50 for MEVB strain by the first and second amplifications respectively. Meanwhile, the first amplification PCR products digested by restriction enzyme could be used to distinguish canine parvovirus and mink enteritis virus, so method has significant useful value and application prospect.

关 键 词：水貂肠炎细小病毒 巢式PCR PCR-RFLP 

分 类 号：S858.33[农业科学—临床兽医学] S858.315.3[农业科学—兽医学]