HMG、EGF和TGF-α对猪卵母细胞体外成熟及发育的影响  

Effect of HMG,EGF,TGF-α on the porcine oocytes maturation in vitro and oocytes development

作　　者：关伟军[1] 梁素丽[1,2] 李向臣[1] 白修云[1,3] 王有柱[1,4] 马月辉[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100094 [2]西北农林科技大学,陕西杨陵712100 [3]山西农业大学,山西太谷030801 [4]宁夏大学,宁夏银川750021

出　　处：《西南农业学报》2008年第6期1719-1723,共5页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科技平台建设项目(2005DKA21101)

摘　　要：以TCM199为基础培养基,HMG(尿促性素)为激素,探讨了HMG在猪卵母细胞体外成熟(IVM)和发育中的影响;同时对EGF(表皮生长因子)和TGF-α(转化生长因子-α)单独或联合使用对猪卵母细胞IVM及发育的影响进行了研究,以确立猪卵母细胞IVM较好的培养体系。结果表明:①在猪卵母细胞IVM中分别添加0.075、0.15、0.2、0.3 IU/mL的HMG,其中添加0.2 IU/mL的HMG组卵母细胞的成熟率和激活后的卵裂率显著高于其它组,差异显著(P<0.05);②添加50 ng/mL的EGF组成熟率和卵裂率高于对照组和添加30、40、60 ng/mL组,差异显著(P<0.05);③添加15 ng/mL的TGF-α组成熟率高于对照组和添加5、40ng/mL组,有显著差异(P<0.05),激活后卵裂率添加15 ng/mL组显著高于其他组(P<0.05),当TGF-α的浓度高于15 ng/mL后对卵母细胞的成熟和激活后的卵裂没有显著的提高作用;④同时添加EGF和TGF-α组成熟率和卵裂率与对照组相比差异显著(P<0.05),但与添加50 ng/mL EGF、15 ng/mLTGF-α组相比没有明显不同(P>O.05)。可见EGF和TGF-α对猪卵母细胞的IVM和激活后的卵裂没有明显的协同作用。TCM199 was used as eultule medium to discuss the affection of HMG as hormone on porcine oooytes maturation in vitro and development. At the same time effects of EGF,TGF-α separation or combination on porcine oocytes IVM and development were studied to establish better culture system. The results showed that ： （i）The maturation rate and the cleavage rate derived from oocytes matured in medium supple- mented with 0.2 IU/mL HMG was significantly higher compared with 0.075,0. 15,0.3 IU/mLHMG groups （P〈0.05 ） ; （ii） The maturation rate and the cleavage rate derived from oocytes matured in medium supplemented with 50 ng/mLEGF was significantly higher compared with control and 30,40,60 ng/mL groups （P〈0.05） ;（iii） The maturation rate derived from oocytes matured in medium supplemented with 15 ng/mLTGF-α was significantly higher compared with 5,40 ng/mL groups（P〈0.05）, The cleavage rate was significantly higher with 15 ng/mLTGF-αthan other groups（ P 〈 0.05 ） ,The maturation rate and the cleavage rate was not highter when the concentration of TGF-α was above 15 ng/mL; （iv） The maturation rate and the cleavage rate derived from oocytes matured in medium supplemented with EGF and TGF-α was significantly higher compared with control（P 〈0.05） ,but it was not significantly difference with 50 ng/mL EGF and 15 ng/mLTGF-α（P〉0.05）. It was showed that EGF and TGF-α did not have cooperate action.

关 键 词：HMG EGF TGF-Α 猪 卵母细胞 体外成熟 

分 类 号：S813.2[农业科学—畜牧学] S828[农业科学—畜牧兽医]