类人胶原蛋白基因工程菌诱导后比生长速率优化  

Optimizing the specific growth rate of recombinant Escherichia coli BL to expressing human-like collagen

作　　者：骆艳娥[1,2] 范代娣[1,2] 米钰[1,2] 马晓轩[1,2] 迟雷[1,2] 王小刚[1,2] 

机构地区：[1]西北大学陕西省可降解生物医用材料重点实验室 [2]陕西省生物材料与发酵工程技术研究中心,陕西西安710069

出　　处：《西北大学学报（自然科学版）》2008年第6期943-946,共4页Journal of Northwest University（Natural Science Edition）

基　　金：“863”计划基金资助项目(2006AA02Z246);国家自然科学基金资助项目(20476085,20606026)

摘　　要：目的优化类人胶原蛋白基因工程菌高温诱导后的比生长速率。方法通过分析不同比生长速率下发酵液中菌体浓度、类人胶原蛋白浓度、细胞得率系数(YX/S)、产物得率系数(YP/S)的变化,确定诱导后的最佳比生长速率。结果当比生长速率为0.04～0.05h-1,细胞浓度和类人胶原蛋白的浓度分别可达69.5g/L(DCW)和13.8g/L。结论诱导后的比生长速率对细胞生长、类人胶原蛋白的合成均产生显著性影响。To optimize the specific growth rate of recombinant Escherichia coli BL expressing human-like collagen after temperature induction. Methods The key parameters, such as biomass, concentration of human-like collagen, cell yield coefficient （ Yx/s ） and product yield coefficient （ Yp/s ）, were analyzed and investigated to optimize the optimal specific growth rates. Results When the specific growth rate was controlled at 0. 04-0. 05 h^-1, the cell concentration and the concentration of human-like collagen could reach 69. 5 g/L （DCW） and 13.8 g/L respectively. Conclusion The specific growth rate of recombinant Escherichia coli BL after induction would affect cell growth and human-like collagen＇s synthesis obviously.

关 键 词：类人胶原蛋白 重组大肠杆菌 温度诱导 比生长速率 

分 类 号：Q939[生物学—微生物学]