桦褐孔菌深层发酵培养条件的优化  

Research on submerged fermentation process of phaeoporus obliquus

作　　者：雷萍[1] 孙悦迎[1] 张文隽[1] 姚树萍 

机构地区：[1]陕西省微生物研究所,陕西西安710043 [2]陕西省农药管理检定所,陕西西安710023

出　　处：《西北大学学报（自然科学版）》2008年第6期967-970,共4页Journal of Northwest University（Natural Science Edition）

基　　金：陕西省科学院重大专项基金资助项目(2007ZD01-03)

摘　　要：目的优化桦褐孔菌深层发酵条件。方法采用L9(34)正交试验法对培养基进行优化。结果桑黄菌体发酵的最优方案是8%葡萄糖,1.60%酵母粉,0.3%KH2PO4,0.15%MgSO4,VB110mg/100mL,pH6.0,接种量为10%,装液量为100mL/300mL三角瓶,150r/min,恒温培养7天,生物量可达1.350g干菌丝每100mL发酵液。结论桦褐孔菌可以用深层液体发酵培养获得菌丝体,桦褐孔菌具有限氧生长的生物学特性。Aim To study the submerged fermentation process and amino acid analysis of Phaeoporus obliquus strain. Methods By adopting L9 （3^4） just cross experimentatiaon method to optimize cuhure medium. Results The composition of the optimal fermentation medium was as fellows ： 8% glucose, 1.60% Powdery yeast, 0. 3% KH2PO4 ,0. 15% MgSO4, VB1 10mg/100mL,pH 6. 0. The optimum cultural period was 7 day in 100/300 mL shake flasks at 150 r/min. The best aeration ratio was 2：5 （medium volume：flask volume）. The inoculation volume was 10%. The highest dry weight of mycelia of Phaeoporus obliquus was obtained about 1. 350 g/150 mL in the optimum culture. Conclusion tation process, Phaeoporus It is feasible that the mycelia of Phaeoporus obliquus were made by submerged fermenobliquus was provided with biological characteristic of limited oxygen.

关 键 词：桦褐孔菌 液体发酵培养 菌丝体 

分 类 号：Q939[生物学—微生物学]