长期酒精摄入引起大鼠胰岛素抵抗及其分子机制  

INSULIN RESISTANCE INDUCED BY CHRONIC ETHANOL INTAKE AND ITS MOLECULAR MECHANISM IN RATS

作　　者：于东[1] 赵立娜[1] 郝丽萍[1] 曲巍 刘烈刚[1] 孙秀发[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院营养与食品卫生系,武汉430030

出　　处：《营养学报》2008年第6期584-587,共4页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.30300282)

摘　　要：目的研究酒精摄入与胰岛素敏感性之间的关系并深入探讨其相关的分子机制。方法清洁级Wistar大鼠80只(雌雄各半),按体重随机分为对照组和低、中、高剂量共4组,每天摄入酒精剂量分别为0、0.8、1.6、2.4g/(kg·bw)。第19w末,断头处死大鼠,测定空腹血糖和血胰岛素,计算HOMA胰岛素抵抗指数(HOMA-IR)。提取肝组织,通过Western blot方法测定磷脂酰肌醇3激酶p85亚单位(p85subunit of phosphoinositide3-kinase,PI-3Kp85),葡萄糖转运体2(Glucose transporter-2)蛋白表达水平。结果与对照组相比,雄性大鼠高剂量组空腹血糖升高,低、中剂量组空腹胰岛素水平升高,各酒精剂量组HOMA-IR指数均升高PI3-K、GLUT-2蛋白在高剂量组表达降低;与对照组比较,雌性大鼠高剂量组空腹血糖升高、空腹血胰岛素下降。各剂量组HOMA-IR指数与对照组比较差异无显著性,PI3-K、GLUT-2蛋白在中,高酒精剂量组的表达均降低。结论长期酒精摄入可以引起胰岛素抵抗,肝脏PI-3K(p85),Glu-4蛋白表达水平的降低,可能是酒精胰岛素抵抗的分子机制之一。Objective To investigate the effect of alcohol intake on insulin sensitivity and its underlying molecular mechanism. Method Eighty Wistar rats were randomly divided into four groups on the basis of body weight, 10 male and 10 female in each. Ethanol was administered at dose of 0 （control group）, 0.8, 1.6, 2.4 g/kg bw （low, medium, high ethanol group, L, M, H） daily. 19 w later, fasting serum glucose and insulin were measured. HOMA-IR index of each group were calculated. The expression of phosphoinosifide 3-kinase （PI-3K, p85）, glucose transporter-2 （GLUT-2） protein in adipose tissue were detected by Western blot. Results In male rats, compared with control group, the fasting plasma glucose of group H, fasting plasma insulin of group L, M and HOMA-IR index of all alcohol-loaded groups were significantly increased. The PI-3K （p85）, and GLUT-2 protein were significantly increased in L and M group, significantly decreased in H group; In female rats, compared with control group, the fasting serum glucose of group H was significantly increased, fasting serum insulin was significantly decreased. HOMA-IR index was insignificantly different in each alcohol-loaded group. The PI-3K （p85）, and GLUT-2 protein were significantly decreased in all alcohol-loaded groups. Conclusion Chronic ethanol intake could induce insulin resistance and the changes of PI-3K, GLUT-2 protein expression level in liver were likely to be the molecular mechanism of alcohol effect on insulin sensitivity.

关 键 词：酒精 胰岛素抵抗 磷脂酰肌醇3激酶 葡萄糖转运体-2 

分 类 号：R587.1[医药卫生—内分泌]