Pb^(2+)对日本三角涡虫急性毒理作用及其6种酶活力的影响  

Acute Toxicity of Pb^(2+) to Dugesia Japonica and Effect of Pb^(2+) on the Activities of Its Six Kinds of Enzymes

作　　者：刘刚[1] 潘红春[1] 王学[1] 王芳芳[1] 范杰[1] 孙祯[1] 

机构地区：[1]安徽师范大学生命科学学院,安徽芜湖241000

出　　处：《安徽师范大学学报（自然科学版）》2008年第6期571-575,共5页Journal of Anhui Normal University(Natural Science)

基　　金："生物环境与生态安全"安徽省高校重点实验室资助项目和安徽省"重要生物资源保护与利用研究"重点实验室资助项目

摘　　要：通过急性毒理实验,观察了Pb2+对日本三角涡虫(Dugesia japonica)的损伤状况,并探讨了Pb2+对涡虫体内LDH、Na+-K+-ATP酶和Ca2+-Mg2+-ATP酶等3种代谢酶和SOD、CAT和GSH-PX等3种抗氧化酶活力的影响.Pb2+对涡虫的24h、36h、48h急性毒理作用半致死浓度(LC50)分别为685.1μmolL-1、402.8μmolL-1、279.2μmolL-1;结果还显示Pb2+浓度越高涡虫的致死时间越短,在相同时间内随着Pb2+浓度的升高涡虫死亡率也随之升高,并且随着Pb2+处理时间的延长涡虫死亡率与培养液中Pb2+浓度的正相关性逐渐提高.另外,Pb2+胁迫对涡虫体内6种酶的活力均有明显的影响.结果表明,日本三角涡虫是一种对Pb2+污染非常敏感的低等动物,在水体Pb2+污染检测等方面具有潜在价值和应用前景.Dugesia japonica was treated with Pb^2＋ . The damage of planarian was observed, and the effect of the Pb^2＋ on activities of lactic dehydrogenase （LDH）, Na^＋ - K^＋ - ATPase, Ca^2＋ - Mg^2＋ - ATPase, superoxide dismutase （SOD）, catalase （CAT） and glutathione peroxidase （GSH- PX ） was studied. The lethal concentration 50（LC50）at 24h, 36h, 48h was respectively 685.1μmolL^-1, 402.8μmolL^-1 and 279.2μmolL^-1. The results indicated that the lethal time of D. japonica became short along with the increasing of Pb^2＋ concentration, and the mortality of planarian was related positively to Pb^2＋ concentration, but the positive relation between the mortality of the planarian and Pb^2＋ concentration was close as the extending of the time when D. japonica was treated with Pb^2＋ . In addition, Pb^2＋ had apparent effect on the activities of six enzymes of D. japonica. Based on the acute toxicity of Pb^2＋ to the planarian and the effect of the activities of its six enzymes, D. japonica should be very sensitive and have great potential value in the testing of Pb^2＋ pollution.

关 键 词：日本三角涡虫 PB^2+ 急性毒理作用 代谢酶 抗氧化酶 

分 类 号：Q959.151[生物学—动物学]