HPLC法测定木豆中牡荆苷和异牡荆苷含量  

Determination of Contents of Vitexin and Isovitexin in Pigeonpea by HPLC

作　　者：佟美鸿[1,2] 刘威[1,2] 付玉杰[1,2] 孔羽[1,2] 顾成波[1,2] 张琳[1,2] 

机构地区：[1]东北林业大学森林植物生态学教育部重点实验室 [2]东北林业大学林业生物制剂教育部工程研究中心,黑龙江哈尔滨150040

出　　处：《林产化学与工业》2008年第6期34-38,共5页Chemistry and Industry of Forest Products

基　　金：国家自然科学基金(30770231);林业科技支撑计划子课题(2006BAD18B0405);教育部科学技术研究重点项目(108049);哈尔滨市科技创新人才研究专项资金(2006RFXXS001)

摘　　要：建立了测定木豆叶、茎、根中牡荆苷和异牡荆苷含量的高效液相色谱分析方法。色谱柱为HIQ Sil C18V(250mm×4.6mm,5μm);流动相为甲醇-水-甲酸(体积比35∶64.74∶0.26);检测波长330nm;流速1mL/min;进样量10μL;柱温30℃。在2～200mg/L牡荆苷、异牡荆苷质量浓度与色谱峰面积线性关系良好;牡荆苷峰面积精密度的相对标准偏差(RSD)最大为2.93%,重复性RSD为2.91%,加样回收率为97.38%;异牡荆苷峰面积精密度的RSD最大为2.69%,重复性RSD为3.37%,加样回收率为98.63%。木豆叶、茎、根中牡荆苷、异牡荆苷的质量分数分别为0.768、0.066、0.183mg/g和0.799、0.139、0.013mg/g。叶中牡荆苷和异牡荆苷含量明显高于茎、根中的含量,可以作为大规模获得牡荆苷、异牡荆苷的资源。A high-performance liquid chromatography （HPLC） method was established for determining contents of vitexin and iso- vitexin in the leaves, stems and roots of pigeonpea （Cajanus cajan（ L. ）Millsp）. The analytes were separated on a HIQ Sil C18V （250 mm x 4.6 mm, 5 μm） column, using methanol-water-formic acid （ 35 ： 64.74 ： 0.26, volume ratio）, as mobile phase, detection wavelength 330 nm, flow rate 1 mL/min, injection volume 10 μL, and column temperature 30 ℃. The calibration curves of vitexin and isovitexin showed good linearity within the ranges of 2-200 mg/L. For vitexin, relative standard deviation （RSD） of precision was not higher than 2.93 %, RSD of repeatability was 2.91%, and recovery was 97.38 % ; for isovitexin, RSD of precision was not higher than 2.69 %, RSD of repeatability was 3.37 %, and recovery was 98.63 %. Mass factions of vitexin and isovitexin in pigeonpea leaves, stems and roots were O. 768, 0. 066, 0. 183 mg/g and 0. 799, 0. 139, 0. 013 rag/g, respectively. Pigeonpea leaves contain much higher amounts of vitexin and isovitexin than stems and roots, which could be developed as a good resource for obtaining vitexin and isovitexin on large scale.

关 键 词：木豆 牡荆苷 异牡荆苷 

分 类 号：TQ351.0[化学工程] Q94[生物学—植物学]