组成型表达线粒体小分子热激蛋白提高番茄抗冷性  

CONSTITUTIVE EXPRESSION OF MITOCHONDRIAL SMALL HEAT-SHOCK PROTEIN ENHANCES CHILLING TOLERANCE IN TOMATO PLANTS

作　　者：王佳颖[1] 张景霞[1] 王翠[1] 杨传燕[1] 孙艳[1] 刘箭[1] 

机构地区：[1]山东师范大学生命科学学院,济南250014

出　　处：《山东师范大学学报（自然科学版）》2008年第4期100-102,共3页Journal of Shandong Normal University(Natural Science)

基　　金：国家自然科学基金资助项目(30270132)

摘　　要：线粒体小分子热激蛋白不但是线粒体的主要热诱导产物,而且也被低温诱导,线粒体小分子热激蛋白可以提高植物的耐热性,但目前还不清楚它是否与植物的耐冷性有关.本实验利用基因工程方法,将番茄线粒体小分子热激蛋白基因(LeHsp23.8)导入番茄,Northern-blotting及Western-blotting分析结果表明,在35sCaMV启动子的驱动下,导入的线粒体小分子热激蛋白基因呈现组成型表达.转基因番茄呈现耐低温性更强的表型,说明组成型表达线粒体小分子热激蛋白基因能提高番茄的抗冷性.The mitochondrial small heat-shock protein is usually the most heat response one of mitoehondrial heat shock proteins, and also can be induced by cold stress, but whether the mitoehondrial small heat-shock protein improves the cold resistance is unknown. By genetic engineering method, the mitochondrial small heat-shock protein gene was introduced into tomato plants. Both Northern-blotting and Western-blotting analyses indicated the constitutive expression of mitochondrial small heat-shock protein driven by 35sCaMV promoter. The transgenic tomato plant showed the improved phenotype of chilling tolerance. The findings suggested that the constitutive expression of the mitochondrial small heatshock protein enhanced the ability of chilling tolerance in tomato plants.

关 键 词：线粒体小分子热激蛋白 抗冷性 转基因番茄 RHODAMINE 123 

分 类 号：Q78[生物学—分子生物学]