大鼠胫骨骨折愈合过程中Ⅰ,Ⅱ型胶原蛋白的表达:失神经状态下Western blot方法测定(英文)  

Expression of type Ⅰ and type Ⅱ collagen in the healing of denervated tibial fracture in rats: A Western blot analysis

作　　者：马骋[1] 高岩[1] 苟三怀[2] 何仿[2] 肖海军[2] 

机构地区：[1]北华大学附属医院骨一科,吉林省吉林市132001 [2]解放军第二军医大学长征医院骨科,上海市200006

出　　处：《中国组织工程研究与临床康复》2008年第50期9978-9981,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:近年来大量的实验及临床观察均证实神经因素对骨折愈合有调节和支配作用。Ⅰ型胶原是促成骨细胞分化和增强成骨细胞黏附能力主要因素,是组成骨构架的基质蛋白;而Ⅱ型胶原由软骨细胞产生。目的:观察失神经状态下骨折愈合过程中Ⅰ,Ⅱ型胶原蛋白表达的变化规律。设计、时间及地点:随机对照动物实验,于2005-05/12在解放军第二军医大学动物实验室及细胞生物教研究室完成。材料:选用3月龄健康雄性SD大鼠40只,采用随机数字表法分为2组,单纯胫骨骨折组与脊髓损伤并胫骨骨折组各20只。方法:单纯胫骨骨折组大鼠于从左胫骨平台前缘插入1根φ0.8mm克氏针,制成胫骨骨折模型。脊髓损伤并胫骨骨折组在植备上述模型的基础上,横断切除T10段脊髓约0.3cm,制成T10脊髓完全性损伤大鼠胫骨骨折模型。主要观察指标:分别于伤后1,2,4,5周采用Western blot法检测两组大鼠骨折断端骨痂中Ⅰ,Ⅱ型胶原的蛋白表达。结果:伤后第1周两组大鼠骨折断端骨痂中Ⅰ,Ⅱ型胶原均有表达,脊髓损伤并胫骨骨折组两种胶原的表达程度均高于单纯胫骨骨折组(P<0.05);伤后第2周脊髓损伤并胫骨骨折组Ⅱ型胶原的表达量达到峰值,高于单纯胫骨骨折组(P<0.05);伤后第4周单纯胫骨骨折组Ⅰ型胶原表达量达峰值,高于脊髓损伤并胫骨骨折组(P<0.05),脊髓损伤并胫骨骨折组Ⅱ型胶原仍有高表达;伤后第5周两组Ⅰ,Ⅱ型胶原表达量均下降。结论:失神经状态下骨折愈合过程中Ⅰ、Ⅱ胶原的分泌规律与正常骨折愈合一致,区别在于各时间点尤其是在峰值点上表达量有明显差异。BACKGROUND： It has been recently indicated that nervous factors are able to adjust and dominate bone fracture healing. Type Ⅰ collagen is a major factor to promote the differentiation of osteoblasts and enhance the adhesion of osteoblasts; while, it is also a matrix protein for composing bone framework. Type Ⅱ collagen is derived from chondrocytes. OBJECTIVE： To study changing law of type Ⅰ and Ⅱ collagen expression during denervated bone fracture healing. DESIGN, TIME AND SETTING： Randomized controlled anima study was performed at the Animal Laboratory and Cell Biology Laboratory, the Second Military Medical University of Chinese PLA between May and December 2005. MATERIALS： Forty 3-month-year healthy male SD rats were randomly divided into fracture group （tibial fracture alone） and combination group （spinal cord injury combined with tibial fracture）, with 20 rats at each group. METHODS： A Φ 0.8 mm Kirschner wire was inserted into anterior border of left tibial plateau to establish tibial fracture models in the fracture group. A 0.3-cm spinal cord transection was cut at T10 segment to establish tibial fracture models with entire spinal cord injury. MAIN OUTCOME MEASRUES： Type Ⅰ and Ⅱ collagen protein expressions of callus were detected using Western blot technique in week 1, 2, 4, and 5 post-injury. RESULTS： One week after injury, type Ⅰ and Ⅱ collagen was represented in callus in the two groups, while the expressions in the combination group were significantly higher than fracture group （P 〈 0.05）; two weeks after injury, typeⅡ collagen expression reached at the peak in the combination group, and the expression was significantly higher than the fracture group （P 〈 0.05）; four weeks after injury, type Ⅰ collagen expression reached at the peak in the fracture group, and the expression was significantly higher than the combination group （P 〈 0.05）, while type Ⅱ collagen still highly expressed in the combination group; five weeks after injury, type Ⅰ

关 键 词：去神经支配 骨折愈合 胶原Ⅰ 型 胶原Ⅱ型 

分 类 号：R681[医药卫生—骨科学]