老鹳草属植物遗传多样性的RAPD分析  

RAPD Analysis for Genetic Diversity of Geranium

作　　者：尹海波[1] 韩荣春[1] 康廷国[1] 王冰[1] 许亮[1] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600

出　　处：《中药材》2008年第12期1788-1790,共3页Journal of Chinese Medicinal Materials

摘　　要：目的:分析8种老鹳草属植物遗传多样性和亲缘关系。方法:利用随机扩增多态性DNA(RAPD)技术分析遗传多样性,UPGMA类平均法聚类分析。结果:筛选出的11个随机引物供试材料DNA随机扩增,共得到145条带。其中多态性条带114条,多态性为78.62%。经聚类分析,可将供试材料分为3类,与传统分类方法差异不大。结论:RAPD技术用于老鹳草属的遗传多样性和分类研究是可行的。Objective ： To analyze the genetic diversity and affinity relationships among 8 species of Geranium. Methods ： Random amplified polymorphic DNA （RAPD） technique was used to analyze genetic diversity, and the dendrogram was constructed by UPGMA. Results ： Eleven RAPD primers were elected for the random amplification. A total of 145 DNA bands were detected, 114 among which were polymorphic, the average rate of polymorphic bands was 78.62%. The result of cluster analysis by using UPGMA method showed that 8 genotypes could be classified into three types. This outcome was corresponding to the traditional classification. Conclusion： It is concluded that RAPD markers can be used on the studies of geneic relationships and classification of species of Gernium sensitively.

关 键 词：老鹳草属 RAPD 遗传多样性 亲缘关系 聚类分析 

分 类 号：R282.77[医药卫生—中药学]