半边旗二萜化合物5F和大黄素影响HO-8910PM细胞中GDF15表达的研究  

Effects of 5F from Pteris semipinnata and Emodin on Expression of GDF15 in HO-8910PM Cell Line

作　　者：何太平[1] 吕应年[2] 龚先玲[2] 莫丽儿[2] 陈功[3] 梁念慈[1] 

机构地区：[1]广东医学院生物化学与分子生物学研究所,广东湛江524023 [2]广东天然药物研究与开发重点实验室,广东湛江524023 [3]香港中文大学威尔斯亲王医院外科学系,中国香港

出　　处：《中药材》2008年第12期1845-1848,共4页Journal of Chinese Medicinal Materials

基　　金：粤港科技合作项目(GHP/022/06);广东省卫生厅课题(B2005104);广东省中医药局课题(1060044)

摘　　要：目的:研究半边旗二萜化合物5F(简称PsL5F)和大黄素对人高转移卵巢癌HO-8910PM细胞中生长分化因子15(GDF15)表达的影响,探讨它们抗肿瘤的作用机制。方法:应用肿瘤基因芯片、荧光定量实时PCR和Western blot法检测PsL5F和大黄素对GDF15表达的影响。结果:PsL5F和大黄素都能明显上调GDF15表达。结论:PsL5F和大黄素的抗肿瘤作用与GDF15高表达有关。Objective：To investigate effects and mechanisms of 5F from Pteris semipinnata （PsL5F） and emodin on expression of growth differentiation factor 15 （GDF15） in highly metastatic ovarian carcinoma HO-8910PM cells. Methods：The expression level of GDF15 was assessed by microarray Chip. Fluorescent quantitative real-time PCR assay and Western blot were performed to verify the effects of PsL5F and emodin on GDF15 expression. Resuhs：PsL5F and emodin significantly up-regulated the expression of GDF15. Conclusion： Antitumor effects and mechanisms of PsL5F and emodin on HO-8910PM ceils are associated with the expression of GDF15.

关 键 词：半边旗二萜化合物5F 大黄素 卵巢癌 GDF15 

分 类 号：R282.5[医药卫生—中药学]