化湿液对湿阻证大鼠胃粘膜损伤修复的影响  

Effects of Huashi Liquid on the Repair to Impaired Gastric Mucosa in Rats with Dampness Retention Syndrome

作　　者：韩志刚[1] 黄学宽[2] 刘群英[2] 朱艺[2] 杨俊卿[3] 

机构地区：[1]重庆医科大学实验动物中心,重庆400016 [2]重庆医科大学中医药学院 [3]重庆医科大学药理学教研室,重庆400016

出　　处：《中药材》2008年第12期1849-1851,共3页Journal of Chinese Medicinal Materials

基　　金：重庆市卫生局科研资助项目(2005-B-37)

摘　　要：目的:研究化湿液对湿阻证模型大鼠胃粘膜损伤修复的影响。方法:将50只SD大鼠随机分为正常组,模型组,化湿液低、中、高剂量组,每组10只。除正常组外,其余4组用改进后的环境加疲劳法制造湿阻证模型,连续造模6d。造模成功后,正常组和模型组大鼠按2 ml/100 g灌胃给予生理盐水;化湿液低、中、高剂量组分别以0.4、0.8、1.6 g生药/100 g灌胃给予化湿液,1次/d,连续8 d后观察各组大鼠胃粘膜损伤情况;取大鼠胃粘膜组织,检测SOD、MDA和NO含量。结果:与正常组比较,模型组大鼠胃粘膜出现充血、水肿,并伴有大量出血点或凝血块,胃粘膜SOD和NO含量显著降低(P<0.05),MDA含量显著增加(P<0.05);与模型组比较,化湿液治疗后湿阻证大鼠胃粘膜充血、水肿和出血等症状完全消失,同时胃粘膜SOD及NO含量增加(P<0.05),MDA含量降低(P<0.05)。结论:化湿液对湿阻证大鼠胃粘膜损伤具有修复作用,其机制可能与提高胃粘膜SOD及NO含量,降低胃粘膜MDA含量有关。Objective ： To investigate the effects of Huashi Liquid on the repair to impaired gastric mueosa in rats with dampness retention syndrome. Methods：Fifty SD rats were randomly divided into normal group, model group, high,middle and low-dose of Huashi Liquid groups, 10 rats in each group. Dampness retention syndrome was induced in all rats,except the normal group,by modified enviromental and fatigued method for 6 days. After the model was formed,the normal and model groups were fed with saline （NS） in 2 ml/ 100 g dose,high,middle and low-dose of Huashi Liquid groups were fed with Huashi Liquid in different dose （0.4 g/100g,0. 8 g/100 g, 1.6 g/100 g） respectively,one time per day,last for 8 days. Then,the impaired extent of gastric mucosa was observed and the content of superoxide dismutase （SOD）, malondialdehyde （MAD） and nitric oxide （NO） in gastric mucosa ware detected. Results：In gastric mucosa of model group, Hyperemia, edema and massive hemorrhagic spots or sludged blood existed, SOD and NO significantly decreased （ P 〈 0. 05 ）, MAD significantly increased （ P 〈 0. 05 ）, compared with the normal group. In gastric mucosa of all the Huashi Liquid, symptoms such as Hyperemia, edema and hemorrhage completely disappeared, SOD and NO significantly increased （ P 〈 0. 05 ）, MDA significantly decreased （ P 〈 0. 05 ）, compared with the model group. Conclusion ： Huashi Liquid could repair gastric mucosa impairment of rats with dampness retention, the mechanism may be related to increasing SOD, MAD and decreasing NO content in gastric mucosa.

关 键 词：化湿液 湿阻证 胃粘膜 超氧化物歧化酶 丙二醛 一氧化氮 

分 类 号：R285.5[医药卫生—中药学]