蒸汽预复水在冻干红细胞复水中的影响及机制  

The influence and the mechanism of the vapor pre-rehydration in the lyophilizing RBCs

作　　者：杨媛媛[1] 舒志全[2] 刘忠 姚余有[1] 

机构地区：[1]安徽医科大学检验医学系,合肥230032 [2]中国科学技术大学热科学与能源工程系,合肥230027 [3]合肥红十字会中心血站,合肥230031

出　　处：《安徽医科大学学报》2008年第6期660-664,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金重点项目(编号:50436030)

摘　　要：目的了解蒸汽预复水对冻干红细胞的保护机制,分析由于渗透压变化造成细胞体积变化而溶血的损伤机制,优化复水程序、降低溶血率。方法将冻干后的红细胞置于37℃培养箱中预复水3h后进行复水,并以未进行预复水的红细胞作为对照,对两组红细胞的形态学、溶血率、ATP含量、2,3-DPG含量及回收率进行评价。结果红细胞溶血率实验组为22.2%±2.1%,对照组为35.4%±2.4%(P<0.05);红细胞2,3-DPG含量实验组为(7.01±1.17)μmol/gHb,对照组为(6.82±2.10)μmol/gHb(P>0.05);红细胞ATP含量实验组为(5.13±0.52)μmol/gHb,对照组为(5.22±0.10)μmol/gHb(P>0.05);与对照组比较,实验组的压积回收率和细胞平均体积明显增高(P<0.05),体积分布宽度明显下降(P<0.05)。结论蒸汽预复水能够保护红细胞膜的完整性,大大提高冻干红细胞的回收率。Objective To study the protection mechainism of the wapor pre-rehydration to the lyophilizing RBCs, analyse the injury of the hemolysis beeaused by the osmotic changes and the volume ehangs of the cells, so as to optimize the rehydration program and reduce the hemolysis rate. Methods The lyophilizing RBCs put in cultivating box at 37℃ for 3 hours were rehydated and the RBCs without pre-rehydatrion were used as control. The two groups were evaluated by the morphology, the hemolysis rate, the ATP level, the 2,3-DPG level and so on. Results The hemolysis rate of pre-rehydatrioned group was （22.2 ± 2. 1 ） % , while the hemolysis rate of the control group was （35.4 ± 2. 4） % （ P 〈 0.05 ） ; The level of 2,3-DPG in RBCs of pre-rehydatrion group was （7.01 ± 1.17 ） umol/ glib,which of the control group was （6. 82 ±2. 10） umol/gHb（P 〉0. 05） ;The level of ATP in RBCs of pre-rehydatrion group was （ 5.13 ± 0. 52 ） umol/gHb, which of the control group was （ 5.22 ± 0. 10 ） umol/gHb （ P 〉 0. 05） ;compared with control group, the recovery of packed cell volume（PCV） and the mean corpuscular volume （MCV） of pre-rehydatrioned group was significantly higher（P 〈0. 05） ,and the RDW was significantly lower（P 〈 0. 05）. Conclusion The pre-rehydatrion can maintain the integrity of RBC membrane, and significantly enhance the recovery of lyophilizing RBCs.

关 键 词：红细胞 冷冻干燥法 

分 类 号：R318.08[医药卫生—生物医学工程] R331.122[医药卫生—基础医学]