免疫性肝损伤大鼠枯否细胞对肝星状细胞增殖的影响及芍药苷的作用  

The effect of kupffer cells on the proliferation of hepatic stellate cells isolated from rats with immunological liver injury and the role of paeoniflorin

作　　者：李响[1] 刘浩[1] 路景涛[1] 魏伟[1] 

机构地区：[1]安徽医科大学临床药理研究所、抗炎免疫药理学省部共建教育部重点实验室、抗炎免疫药物安徽省工程技术研究中心,合肥230032

出　　处：《安徽医科大学学报》2008年第6期679-682,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:30572356);安徽省科技攻关计划项目(编号:06013133B);高等学校博士学科点专项科研基金(编号:20060366003);安徽高校首批科技创新团队基金(编号:2005TD003);安徽省研究实验基地优秀中青年科研带头人基金(编号:200622805)

摘　　要：目的探讨芍药苷(Pae)通过免疫性肝损伤大鼠枯否细胞(KC)影响肝星状细胞(HSC)的增殖能力。方法用卡介苗加脂多糖诱导大鼠肝损伤模型,肝脏原位灌流分离大鼠KC和HSC,观察共培养的形态学改变,观察Pae作用后的KC培养上清对HSC增殖的影响。结果免疫性肝损伤大鼠KC能明显促进HSC的增殖,在Pae作用后,KC培养上清能抑制HSC的增殖。结论KC是Pae发挥作用的靶细胞之一,Pae可通过作用于KC抑制HSC的增殖。Objective To investigate the effects of kupffer cell（KC） culture medium on the proliferation of hepatic stellate cells （HSC） isolated from rats with immunological liver injury and the role of paeoniflorin. Methods Rats were induced by Bacillus Calmette-Guerin plus lipopolysaccharide, KC and HSC were isolated by means of liver perfusion in suit. The morphology changes in co-cluture were observed on the 7th day. The effects of KC culture medium supplemented with different concentrations of paeoniflorin on HSC proliferation were detected by MTT assay. Results KC supernatants could promote the HSC proliferation. KC supernatants treated with paeoniflorin could decrease the promoting effects of KC on HSC proliferation. Conclusion Paeoniflorin can decrease effects of KC on promoting HSC proliferation via modulation for KC function.

关 键 词：枯否细胞 细胞增殖 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R575[医药卫生—基础医学]