慢性乙型肝炎病毒感染者转化生长因子-β1基因单核苷酸多态性分析  

Analysis of single nucleotide polymorphism of the transforming growth faetor-β1 gene in patients with chronic hepatitis B virus infection

作　　者：龚邦东 裴彬 杜德林 李仲平 李宇舟 

机构地区：[1]上海市南汇区南华医院感染科,201300

出　　处：《中华传染病杂志》2008年第12期725-728,共4页Chinese Journal of Infectious Diseases

摘　　要：目的探讨转化生长因子（TGF）-β1单核苷酸多态性（SNP）与慢性HBV感染的关系。方法采用PCR方法，对74例慢性乙型肝炎患者、41例乙型肝炎肝硬化患者和41例健康人外周血细胞基因组DNA的TGF-β1基因中三个SNP位点（rs2241715、rs2241716、rs4803455）进行基因分型。数据分析采用X2或Fisher精确概率法检验。结果健康人的rs2241715基因型及等位基因频率与慢性乙型肝炎和肝硬化患者相比，差异有统计学意义（X2=11．419，P〈0．01或X2=6．218，X2=5．96]；P〈O．05），rs2241715T／T基因型的个体患慢性乙型肝炎和乙型肝炎肝硬化的相对危险度分别为其他型的2．974倍（95％CI=1．209～7．314，P=0．018）和3．228倍（95％CI=1．201～8．675，P=0．020），rs2241716和rs4803455位点则无上述相关性。结论TGF-β1基因的rs2241715SNP位点T／T基因型可能与慢性HBV感染密切相关。Objective To explore the genetic impact of three newly discovered single nueleotide polymorphism （SNP） sites of the transforming growth factor （TGF）-β1 gene on the susceptibility of the chronic hepatitis B virus （HBV） infection. Methods Genome DNA was extracted from the peripheral blood samples of 115 cases suffering from the chronic HBV infection （74 chronic hepatitis B, 41 cirrhosis） as well as 41 healthy volunteers. Thereafter, genotyping of rs2241715, rs2241716 and rs4803455 sites of the TGF-131 gene was performed by genotype-speeific polymerase chain reaction （PCR） analysis. The data were analyzed by the ehi square test and Fisher exact test. Results There was a significant difference of rs2241715 genotypes and allele frequencies between healthy volunteers 2 and patients with chronic hepatitis B and cirrhosis （X2 = 11.419, P〈0.01 and X2 = 6.218, X2 = 5.961, P〈0.05,respectively）. Interestingly, the risk relative of subjects with T/T genotype suffered from chronic hepatitis B （OR = 2. 974, 95% CI = 1. 209 - 7. 314, P = 0. 018） and cirrhosis （OR = 3. 228, 95%CI=1. 201-8. 675, P=0. 020） was dramatically higher than that in patients with T/G or G/G genotypes. Conclusion The TGF-β1 rs2241715 T/T genotype appears to be associated with the chronic HBV infection.

关 键 词：转化生长因子Β 多态性 单核苷酸 肝炎 乙型 慢性 基因型 聚合酶链反应 

分 类 号：R512.62[医药卫生—内科学] R73[医药卫生—临床医学]