新生鼠坏死性小肠结肠炎肠细胞凋亡率变化及意义  

Change of Intestinal Cells Apoptosis in Neonatal Rat Model with Necrotizing Enterocolitis and its Significance

作　　者：董海英[1] 刘健[1] 陈兢芳[1] 卓玲[1] 杨燕珍[1] 吴斌[1] 

机构地区：[1]福建医科大学附属第一医院儿科,福州350005

出　　处：《福建医科大学学报》2008年第6期503-506,共4页Journal of Fujian Medical University

基　　金：福建省卫生厅青年科研课题(2007-1-10)

摘　　要：目的探讨新生鼠坏死性小肠结肠炎(NEC)肠细胞凋亡率变化及其与肠组织损伤程度的关系。方法出生48h的SD大鼠16只随机分成模型组和对照组,每组8只。模型组给予鼠乳代用品人工喂养,100%氮气缺氧90s,4℃冷刺激10min,每天2次,连续3d,建立NEC模型;对照组与母鼠同笼,鼠乳喂养,未进行缺氧冷刺激。实验结束后24h处死大鼠,留取十二指肠下端至直肠上端肠道组织进行肠组织损伤评分和肠细胞凋亡率检测。每组随机抽取2例回盲部近端小肠进行肠黏膜透射电镜检查。结果模型组肠组织学改变与NEC时改变一致,透射电镜显示肠黏膜出现大量凋亡细胞,部分形成凋亡小体。模型组和对照组肠组织损伤评分分别为(3.33±0.59)和(0.13±0.17);肠细胞凋亡率分别为(31.0±10.6)%和(4.6±3.9)%;二者比较差别均有统计学意义。肠细胞凋亡率与肠组织损伤评分呈显著正相关(r=0.771,P<0.01)。结论NEC时肠细胞凋亡明显增加。肠细胞凋亡可能是造成新生鼠NEC肠道进行性损伤的病理基础。Objective To investigate the change of intestinal cells apoptosis in NEC and the correlation between it and the pathological changes of intestinal tissues. Methods 16 neonatal rats were randomly divided into model group and normal control group （n=8 rats each group）. NEC model was established as follows： neonatal rats were separated from mother rats, fed with rat milk substitute, and given hypoxia（100%N2） for 90 seconds and 4 ℃ cold exposure for 10 minutes, twice a day during 3 consecutive days. On the 4th day all the subjects were sacrificed and intestinal tissues obtained to examine. The intestines around ileocecal junction were chosen from 2 samples of each group for histological evaluation by score and electron microscopic observation. The remaining was used to detected the apoptosis rate of intestinal cells（flow cytometer）. SPSS 11.0 software for Windows was adopted in all statistical tests, α =0.05 was considered significant. Results Transmission electron microscopy showed a large number of apoptotic cells in the intestinal mucosa, and some formatted apoptotic bodies. The scores of histological evaluation（x±s） in experimental group and control group were 3.33±0.59, 0. 13±0. 17, respectively （H = 11. 761, P〈0. 001）. The apoptosis ratio of intestinal cells （%） significantly increases in NEC and apoptosis probably induces intestinal progressive damage on a pathological basis. The scores of histological evaluation was significant positive correlated with the apoptosis rates of intestinal cells（rapoptosis rate = 0. 771, P〈0. 001）. Conclusion The apoptosis rate of intestinal cells significantly increases in NEC and apoptosis probably induces intestinal progressive damage on a pathological basis. The abnormal apoptosis of intestinal cell can increase the permeability of the intestinal epithelium, activate the bacterial translocation and the inflammatory cascades that do harm to organism.

关 键 词：小肠结肠炎 伪膜性 婴儿 新生 疾病 疾病模型 动物 细胞凋亡 

分 类 号：R722.1[医药卫生—儿科]