西洛他唑抗动脉粥样硬化的作用机制  

The Study of Anti-Atherosclerotic Mechanism of Cilostazol

作　　者：卢振华[1] 马业新[1] 冯达应[1] 柯辉[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院心内科,湖北省武汉市430030

出　　处：《中国动脉硬化杂志》2008年第11期881-884,共4页Chinese Journal of Arteriosclerosis

摘　　要：目的观察西洛他唑对兔动脉粥样硬化斑块组织的影响,进一步探讨西洛他唑抗动脉粥样硬化作用及其可能机制。方法将30只新西兰雄性白兔随机分为正常对照组、高脂饮食组和西洛他唑组。酶法检测血脂,免疫沉淀法测定血清C反应蛋白水平,免疫组织化学检测基质金属蛋白酶9和核因子κB的表达,病理形态学分析各组主动脉内膜厚度和斑块面积,逆转录聚合酶链反应检测血管组织单核细胞趋化蛋白1mRNA的表达。结果实验第12周末,西洛他唑组总胆固醇、甘油三酯和低密度脂蛋白胆固醇水平低于高脂饮食组,但高于正常对照组(P均<0.01);西洛他唑组主动脉内膜厚度和斑块面积低于高脂饮食组,但大于正常对照组(P均<0.01);西洛他唑组核因子κB、单核细胞趋化蛋白1和基质金属蛋白酶9的表达弱于高脂饮食组,但强于正常对照组(P均<0.01)。结论西洛他唑有抗动脉粥样硬化作用,其作用机制可能与下调核因子κB、单核细胞趋化蛋白1及基质金属蛋白酶9的表达有关。Aim To observe the effects of Cilostazol on rabbit atherosclerotic plaque and try to illustrate the anti- atherosclerotic mechanism of Cilostazol. Methods Thirty New Zealand male rabbits were randomly divided into normal control group （ n = 10）, high-cholesterol group （ n = 10 ） and Cilostazol group （ n = 10 ）. The levels of serum total cholesterol （TC）, triglyceride （TG） and low density lipoprotein cholesterol （LDLC） were detected by Enzymatic assay,and the level of C-reactive protein （CRP） were detected by immuno-preeipitation; the aortic intimal thickness and plaque area of aorta were measured by pathomorphology; the expression of nuclear factor-KB （NF-KB） and matrix metalloproteinase-9 （ MMP-9 ） was detected with immunohistochemical method, and the expression of monocyte chemoattractant protein-1 （ MCP-1 ） was detected by RT-PCR. Results At the end of 12 weeks, the levels of TC, TG and LDLC in the Cilostazol group were significantly lower than that of high-cholesterol group （ P〈0.01 ）, but higher than that of control group （ P〈 0.01 ） ; the aortic plaque area and intimal thickness in the Cilostazol group were significantly lower than that in the high- cholesterol group （ P 〈 0.01 ）, but higher than that in the control group （ P 〈 0.01 ） ; the NF-κB, MCP-1 and MMP-9 expression of Cilostazol group were sigffificantly lower than that of high-cholesterol group （ P〈0.01 ） , but higher than that of control group （ P 〈 0.01 ）. Conclusion Cilostazol has obvious anti-atherosclerotic effects; reducing the expression of NF-κB, MCP-1 and MMP-9 may be the anti-atherosclerotic mechanism of Cilostazol.

关 键 词：内科学 动脉粥样硬化 西洛他唑 单核细胞趋化蛋白.1 基质金属蛋白酶9 

分 类 号：R5[医药卫生—内科学]