IFN-α联合As_2O_3对NB4细胞株VEGF及Survivin表达的影响  

Effects of interferon-α combining with arsenic trioxide on the expression of VEGF and Survivin in NB4 cell line

作　　者：王树叶[1] 段丽祥 曹峰林[1] 杨喜晶[1] 

机构地区：[1]哈尔滨医科大学第一临床医学院血液科,黑龙江哈尔滨150001 [2]山西省运城市中心医院,山西运城044200

出　　处：《哈尔滨医科大学学报》2008年第6期605-608,共4页Journal of Harbin Medical University

基　　金：哈尔滨市科学研究基金项目(2005AA9CS116-4);黑龙江省卫生厅医学科研基金(2003-035)

摘　　要：目的探讨IFN-α联合不同浓度As2O3对NB4细胞株血管内皮生长因子(VEGF)、凋亡抑制蛋白(Sur-vivin)表达的影响。方法实验分不同浓度的As2O3即0、0.1、0.5、1.0、2.0μmol/L及不同浓度As2O3联合IFN-α共10组,利用MTT法检测细胞毒性反应,同时采用免疫组织化学方法半定量分析NB4细胞VEGF、Survivin表达的动态变化。结果Survivin在未加药组阳性率为93.8%,VEGF在未加药组阳性率为82.4%。As2O3(≤1.0μmol/L)作用后VEGF表达增加,但无统计学意义;Survivin表达下降(P<0.029)。2.0μmol/L As2O3作用下两者表达均明显降低(P=0.006;P=0.000);联合IFN-α组两者表达均明显降低(P=0.000),分析两者呈正相关(r=0.8754)。结论IFN-α联合As2O3可降低VEGF、Survivin的表达,提示IFN可以降低As2O3对NB4细胞凋亡抑制基因的表达,具有调控细胞凋亡的作用。Objective To investigate the expressions of vascular endothelial growth factor （VEGF） and Survivin in NB4 cells lines affected by interferon-α combining with arsenic trioxide. Methods NB4 cells line were treated with arsenic trioxide alone （with doses of 0,0.1 i0.5,1.0,2.0 μmol/L）or in combination with interferon-α. Cytotoxic reaction wasassessed by MTT assay. The dynamic expressions of VEGF and Survivin were detected by semi-quantitative immunohistochemistry. Results The positive rates of VEGF and Survivin in control group were 82.4% and 93.8.% ,respectively. VEGF expression increased under effect of arsenic trioxide （ P〈 1.0 μmol/L） （no statistically significance） , instead, Survivin decreased （P 〈 0.029）. VEGF and Survivin decreased when treating with 2.0 μmoL/Larsenic trioxide, as well as combining with interferon-α. Positive correlation was observed between of them（ r =0.8754）. Conclusion Interferon-α combining with arsenic trioxide can down regulate the expressions of VEGF and Survivin and suggests that interferon-or can decrease the expression of inhibitor of apoptosis gene in NB4 cell line and play a role in the regulation of cell apoptosis.

关 键 词：三氧化二砷 IFN-Α 存活蛋白 血管内皮生长因子 NB4细胞株 

分 类 号：R979.1[医药卫生—药品]