Nestin、Wnt1和β-catenin在小鼠SVZa-RMS-OB通路的表达  

Expressions of Wnt1,Nestin and β-catenin in the region of SVZa-RMS-OB in mice

作　　者：张治元[1] 杨辉[2] 邱克军[2] 刘仕勇[2] 何家全[2] 

机构地区：[1]南京大学医学院临床学院(南京军区南京总医院)神经外科,江苏南京210002 [2]第三军医大学新桥医院神经外科,重庆400037

出　　处：《医学研究生学报》2008年第12期1239-1241,1245,I0011,共5页Journal of Medical Postgraduates

基　　金：国家自然科学基金重点课题(批准号:30130110)

摘　　要：目的:研究Nestin、Wnt1和β-catenin在小鼠SVZa-RMS-OB通路的表达情况。方法:取P0昆明小鼠全脑组织,冰冻切片,切片厚度为20μm,行免疫荧光双标检测Nestin和Wnt1在小鼠SVZa-RMS-OB通路的表达情况,行免疫荧光单标检测β-catenin小鼠SVZa-RMS-OB通路的表达情况。结果:Nestin在SVZa表达最高,在OB表达最低,在RMS表达介于SVZa和OB之间;Wnt1在OB表达最高,在RMS表达最低,在SVZa的表达介于OB和RMS之间;β-catenin在SVZa表达最高,在OB表达最低,在RMS表达介于SVZa和OB之间。结论:随着SVZa神经干细胞从SVZa向OB迁移,Nestin和β-catenin表达逐渐减低;Wnt1在迁移终点OB高表达,在RMS迁移途中表达最低;Nestin、Wnt1和β-catenin在小鼠SVZa-RMS-OB通路的不同表达可能与SVZa神经干细胞增殖、分化有关。Objective： To investigate the expressions of Nestin, Wntl and β-eatenin in the region of SVZa-RMS-OB in mice. Methods ： The whole brain of the postnatal day 0 （P0） Kunming mouse was separated, and the frozen brain cut into 20μm sections. The expressions of Nestin and Wntl in the SV- Za-RMS-OB were detected by double immunofluorescence staining, and that of β-catenin determined by single immunofluorescence staining. Results： The expression of Nestin was the highest in SVZa and lowest in OB; the expression of Wntl was the highest in OB and lowest in RMS; and that of β-catenin was the highest in SVZa and lowest in OB. Conclusion ： With the migration of neural stem cells from SVZa to OB, the expressions of Nestin and β-catenin were gradually decreased. The highest expression of Wntl was observed in OB, the terminal of the migration, and the lowest in the RMS during the migration. The difference in the expression of Nestin, Wntl and β-catenin in the SVZa-RMS-OB of mice might be correlated with the proliferation and differentiation of SVZa neural stem cells.

关 键 词：NESTIN WNT1 Β-CATENIN SVZA RMS OB 

分 类 号：R322.85[医药卫生—人体解剖和组织胚胎学]