变应性鼻炎辅助性T细胞免疫球蛋白功能区和黏液素样功能区-1的表达  

Immunoglobulin domain and mucin domain-1 in helper T lymphocytes in allergic rhinitis

作　　者：武文魁[1,2] 安云芳[1] 赵长青[1] 

机构地区：[1]山西医科大学第二附属医院耳鼻咽喉科,太原030001 [2]天津泰达医院

出　　处：《中华医学杂志》2008年第48期3392-3396,共5页National Medical Journal of China

基　　金：基金项目：国家自然科学基金资助项目（30350006）;国家科技支撑计划基金资助项目（2007BAI18815）;山西省自然科学基金资助项目（2006011115）;山西省资助回国留学人员科研项目（2005032）

摘　　要：目的探讨人辅助性T细胞（helper T lymphocyte，Th）表达T细胞免疫球蛋白功能区和黏液素样功能区-1（T cell immunoglobulin domain and muein domain-1，TIM-1）在变应性鼻炎（allergicrhinitis，AR）中的作用。方法密度梯度离心分离20名健康人和20例AR患者外周血单个核细胞（human peripheral blood mononuclear cells，PBMC），免疫磁珠分选CD4＋T细胞，CD4＋CD45RA＋T细胞，免疫荧光和流式细胞仪检测TIM-1阳性细胞。对比植物血凝素（phytohemagglutinin，PHA，20μg/ml）非特异性刺激上述细胞后表达TIM-1阳性细胞的差异。标准变应原（屋尘螨，50000 SQU／m1）和内毒素（脂多糖，lipopolysaccharide，LPS，20ng／ml）特异性刺激树突状细胞（dendritic cells，DC），观察DC提最抗原并诱导初始Th细胞表达TIM-1的特点。酶联免疫吸附实验（ELISA）检测培养液中IL4、IFN-1的分泌。结果AR患者组CD4＋T细胞中TIM-1阳性CD4＋T细胞9．48％±1．51％，高于健康组3．12％±0．32％（P〈0．05）；PHA刺激后两绀表达TIM-1的CD4＋T细胞均高于刺激前（P值均〈0．05）。DC提呈抗原刺激健康组初始Th细胞后，检测混合细胞液中表达TIM-1的细胞，标准变应原组10．81％±1．48％，高于LPS组6．07％±1．45％（P〈0．05）。ELISA检测相应IL-4、IFN-γ的分泌，标准变应原组IL4为（74．61±13．82）pg／ml、（25．31±7．23）pg／ml；LPS组分别为（28．57±3．36）pg／ml、（163．41±82．37）pg／ml。结论AR患者中表达TIM-1的Th细胞高于健康者，DC提呈抗原后可以促进其表达；TIM-1的表达有利于初始Th细胞向Th2分化，由此诱发以Th2细胞凼子为主的免疫反应。Objective To investigate the significance of T cell immunoglobulin domain and mucin domain-1 （TIM-1） expression in helper T lymphocyte in allergic rhinitis （AR） patients. Methods Peripheral blood samples were collected from 20 AR patients, 9 males and 11 females, aged 31.4 （20 - 54）, and 20 healthy persons, 12 males and 8 females, aged 38,6 （18 -60）. Peripheral blood mononuclear cells （PBMNs） were isolated and magnetic cell sorting technique was used to purify the CIM ＋ T cells and CIM ＋ CD45RA ＋ T cells. Immunofluorescence staining and flow cytometry were used to detect the percentage of CIM ＋ CD45RA ＋ T cells positive in TIM-I, a helper T lymphocyte marker. Phytohemagglutiniun （PHA） was added into the culture media to stimulate the TIM-1 positive helper T lymphocytes. Dendritic cells （DCs） thus induced and differentiated were conditioned by standardized allergen from dust mite （Demmtophagoides pteronyssinus） or lipopolysaccharide （LPS）, and then cocultured with CD4 T cells. The percentage of TIM-I positive CD4 ＋ CD45RA ＋ T cells was counted. ELISA was used to detect the IL-4 and interferon （IFN） -γ levels in the supernatants of the culture media of the dust mite and LPS groups. Results The percentage of TIM-1 positive CD4 ＋ T cells of the AR patients was （9.48 ＋ 1. 51 ）%, significantly higher than that of the healthy controls [ （3.12± 0. 32 ）%, P 〈 0. 05], and the percentage of TIM-1 positive CD4 ＋ T cells after PHA stimulation of the AR patients was （47. 05 ± 4. 35） %, significantly higher than that of the healthy controls [ （ 29. 92 ± 3.36 ） %, P 〈 0. 05 ]. The percentage of TIM-1 positive CD4 ＋ CD45RA ＋ T cells of the AR patients was （ 26. 34 ± 4. 0 ） %, significantly higher than that of the healthy controls [ （ 18.4 ±8.1 ） %, P 〈 0.05 ], and the percentage of TIM-1 positive CD4 ＋ CD45RA ＋ T ceils after PHA stimulation of the AR patients was （65.3 ± 7.4）%, significantly higher than that of the health

关 键 词：鼻炎 变应性 辅助性T细胞 螨 T细胞免疫球蛋白功能区和黏液素样功能区-1(TIM-1) 

分 类 号：R686[医药卫生—骨科学]