日本血吸虫不同阶段抗原免疫抑制过敏性哮喘小鼠气道炎症的实验观察  

Suppression Effect of Different Stage Antigens of Schistosoma japonicum on Airway Inflammation in a Murine Model of Asthma

作　　者：蒋自卫[1] 莫红梅[1,2] 王磊[1] 程喻力[1] 王淇泓[1] 雷家慧[1] 刘文琪[1] 李雍龙[1] 

机构地区：[1]华中科技大学同济医学院病原生物学系,武汉430030 [2]新疆维吾尔自治区人民医院检验科,乌鲁木齐830000

出　　处：《中国寄生虫学与寄生虫病杂志》2008年第6期428-431,437,共5页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：目的观察日本血吸虫不同生活史阶段抗原对过敏性哮喘小鼠气道炎症的影响。方法雌性BALB/c小鼠48只,随机均分8组,A组为健康对照组;B组为哮喘模型组,以鸡卵白蛋白(OVA)抗原腹腔注射致敏,OVA滴鼻诱发哮喘;C、D和E组小鼠分别用可溶性虫卵抗原(SEA)、可溶性成虫抗原(SWA)和童虫抗原(SSA)经腹部皮下免疫,共4次,每次间隔1周,末次免疫后1周再按B组方法诱发哮喘;F、G和H组分别用SEA、SWA和SSA免疫接种小鼠(免疫方法及剂量分别同C、D、E组),末次免疫后1周用等量生理盐水代替OVA处理小鼠,不诱发哮喘。诱发哮喘后48h剖杀各组小鼠,观察各组小鼠肺组织的病理变化和支气管肺泡灌洗液(BALF)中的相关细胞成分及细胞因子的变化。结果A组小鼠气道肺组织无明显炎症变化;B组小鼠气道肺组织可见明显的炎性细胞,尤其是嗜酸粒细胞的浸润;C、D和E组小鼠气道肺组织炎症明显轻于B组小鼠。B组小鼠BALF中白细胞总数[(98.4±16.1)×104/ml]、嗜酸粒细胞百分数[(17.6±4.3)×104/ml]和白细胞介素-5(IL-5)水平[(197.9±36.5)pg/ml]均明显高于A组[分别为(8.2±1.1)×104/ml、(0.02±0.01)×104/ml和(12.3±7.4)pg/ml],而IL-10和γ干扰素(IFN-γ)水平明显低于A组。C、D和E组小鼠BALF中白细胞总数、嗜酸粒细胞百分数和IL-5水平均明显低于B组,而IL-10,IFN-γ水平则明显高于B组,差异有统计学意义(P<0.05)。结论日本血吸虫不同抗原免疫后能有效调节过敏性哮喘小鼠的细胞因子平衡,同时对哮喘小鼠嗜酸粒细胞在肺组织中的浸润及肺组织炎症都有一定的抑制作用。Objective To investigate the effect of antigens of different stage Schistosoma japonicum on airway inflammation in a murine model of asthma. Methods 48 female BALB/c mice were randomly divided into eight groups. Mice in group A were given normal saline of equal volume as control. Group B was asthma model which was established by intraperitoneal and intranasal challenge with OVA. Mice in groups C, D and E were immunized with soluble egg antigen （SEA）, soluble male worm antigen （SWA）, and schistosomulum antigen （SSA） respectively 4 times in a week interval, followed by OVA sensitization as in group B 1 week after the final immunization. Mice in groups F, G, and H were immunized with SEA, SWA, and SSA respectively but sensitized and challenged with saline instead of OVA. 48 hours after asthma was induced, the mice were sacrificed. Leukocytes and eosinophils were counted in bronchoalveolar lavage fluid （BALF）. The level of IL-5, IL-10 and IFN-γ in BALF was detected. Pathologic changes in lung tissues were observed. Results Inflammation cells, especially eosionphils, appeared in airways of mice in groups B, C, D and E, but with much less number in groups C, D and E. No inflammation cells were seen in airways of group A mice. The number of leukocytes, eosinophils and level of IL-5 in BALF of group B [（98.4±16.1） ×10^4/ml, （17.6±4.3） ×10^4/ml, （197.9±36.5） pg/ml respectively] were significantly higher than those of group A [（8.2±1.1）×10^4/ml, （0.02±0.01） ×10^4/ ml, （12.3±7.4） pg/ml], however the levels of IL-10 and IFN-γ were significantly lower than that of group A （P〈0.05）. The number of leukocytes, especially eosinophils, in BALF of groups C, D and E was significantly lower than that of group B. The level of IL-5 in BALF of groups C, D and E was significantly reduced, while that of IL-10 and IFN-γ in BALF of the 3 groups was significantly higher than group B （P〈0.05） . Conclusions The immunization with S. japonicum antigens can effectively modulate the

关 键 词：日本血吸虫 抗原 哮喘 细胞因子 

分 类 号：R383.24[医药卫生—医学寄生虫学]