磷酸萘酚喹伍用青蒿素对猴疟原虫的药效学研究  

Therapeutic Efficacy of Naphthoquine Phosphate Combined with Artemisinine against Plasmodium knowlesi

作　　者：王京燕[1] 丁德本[1] 李国福[1] 赵京花[1] 

机构地区：[1]军事医学科学院病原微生物生物安全国家重点实验室,北京100071

出　　处：《中国寄生虫学与寄生虫病杂志》2008年第6期442-444,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：军队医药卫生科研基金(No.9108035-39)~~

摘　　要：目的研究磷酸萘酚喹与青蒿素伍用对猴疟的治疗作用。方法感染诺氏疟原虫恒河猴模型随机均分为9组(3只/组),A组和B组分别灌服6和10mg/kg磷酸萘酚喹3d,1次/d;C组和D组分别灌服31.6和100mg/kg青蒿素,第1天2次,第2～3天各1次;E组、F组和G组用10mg/kg磷酸萘酚喹分别与10、20和25mg/kg青蒿素配伍(即以1誜1、1誜2和1誜2.5配伍)灌服;H组和I组分别灌服单药磷酸萘酚喹10mg/kg和青蒿素30mg/kg。于给药后24h观察原虫感染率,以给药后105d查不见原虫为治愈标准。结果给药后24h,A、B、C、D组原虫下降率均超过90%。E、F和G组平均原虫转阴时间依次为(56.0±16.0)、(53.3±4.6)和(56.0±8.0)h,均较H组[(69.3±4.6)h]快。A、B、D、E、F和G组治愈猴数分别为1、3、3、2、2和3只。C、H和I组均未治愈。结论磷酸萘酚喹与青蒿素伍用可降低伍用单药剂量,缩短疗程,提高治愈率,两药1誜2.5的比例配伍治愈率达100%。Objective To study the antimalarial activity of naphthoquine phosphate combined with artemisinine against Plasmodium knowlesi in rhesus monkey. Methods Monkeys were randomly divided into 9 groups （3/group）. The monkeys in groups A and B were treated i.g. once daily for 3 days with 6 or 10 mg/kg of naphthoquine phosphate respectively. Those in groups C and D were treated i.g. twice for the 1st day and once for the 2nd and 3rd day with 31.6 or 100 mg/kg of artemisinine respectively. In groups E, F and G, they were treated i.g. only once with the combination of naphthoquine phosphate 10 mg/kg and artemisinine 10, 20 or 25 mg/kg respectively. Groups H and I served as controls which were treated i.g. only once with 10 mg/kg of naphthoquine phosphate and 30 mg/kg of artemisinine respectively. Parasitemia was examined beginning 24 h after drug administration. The observation lasted 105 days when no more parasite was found. Results At 24 h after drug administration, the parasite reduction rate in all groups was higher than 90%. The parasite clearance time for groups E, F and G was （56.0±16.0）, （53.3±4.6）, and （56.0±8.0） h respectively, more rapid than that of Group H [（69.3±4.6） hi. There were 1, 3, 3, 2, 2, and 3 monkeys in groups A, B, D, E, F, and G respectively which were cured. No monkeys were cured in groups C, H and I. Conclusion The combination of naphthoquine phosphate and artemisinine is superior to the single component and the optimum proportion in the combination is 1 ： 2.5 in treating P. knowlesi infection in monkeys.

关 键 词：磷酸萘酚喹 青蒿素 诺氏疟原虫 猕猴 

分 类 号：R513.39[医药卫生—内科学]