Influence of different Fe sources and concentrations on formation of magnetosomes in Acidithiobacillus ferrooxidans  

作　　者：刘文斌 武海艳 刘学端 刘新星 

机构地区：[1]Department of Bioengineering,School of Minerals Processing and Bioengineering,Central South University

出　　处：《中国有色金属学会会刊：英文版》2008年第6期1379-1385,共7页Transactions of Nonferrous Metals Society of China

基　　金：Project(50774102)supported by the National Natural Science Foundation of China;Project(2004CB619201)supported the National Basic Research Program of China;Project(1343-77341)supported by Innovative Group of Education of Graduation Student of Central South University,China

摘　　要：紧张 Acidithiobacillus ferrooxidans ATCC 23270 的整个染色体被生物信息学分析，在 magnetotactic 细菌的功能的一些相当或相同的事物基因是可得到的。为了响应不同 Fe 来源和集中，获得 Acidithiobacillus ferrooxidans 的 magnetosomes 形成机制的进一步的知识，时间的基因表示 mpsA 介绍， magA， mamB 并且你被使用实时量的反向的 transcription-PCR 检验。magnetosomes 和 magnetotaxis 的微生物引起的形成也在不同 Fe2+ 集中下面被学习。结果显示 1622 ATCC 23270， 0276， 1124 和 2572 的 ORF 数字与 mpsA 是相应的，你，在 magnetotactic 细菌的 magA 和 mamB 基因。mpsA， magA 和 mamB 的表示层次是直接在 150200 点与铁的集中，和最高的表示有关铺平 mmol/L 铁被获得。在 ATCC 23270 和房间的 magnetotaxis 的 magnetosomes 的数字是显著地这三基因与表示有关铺平，建议这些基因在在 A 的 magnetosome 形成的过程期间与铁运输是相关的。ferrooxidans。The whole genome of strain Acidithiobacillus ferrooxidans ATCC 23270 was analyzed by bioinformatics and some homolog genes of functional ones in magnetotactic bacteria were available. To obtain further knowledge of the magnetosomes formation mechanisms of Acidithiobacillus ferrooxidans in response to different Fe sources and concentrations, temporal gene expression profiles of mpsA, magA, mamB and thy were examined by using Real-time quantitative reverse transcription-PCR. The microbial formation of magnetosomes and magnetotaxis was also studied under different Fe^2＋ concentration. The results indicated that ORF numbers of 1622, 0276, 1124 and 2572 of the ATCC 23270 were homologous with mpsA, thy, magA and mamB genes in magnetotactic bacteria. The expression levels of mpsA, magA and mamB were directly related to ferrous concentration, and the highest expression level at 150-200 mmol/L ferrous was gained. The number of magnetosomes in ATCC 23270 and the magnetotaxis of cells were significantly related to the expression level of these three genes, suggesting that these genes were related with the iron transport during the process of magnetosome formation in A. ferrooxidans.

关 键 词：氧化亚铁 生物技术 PCR 转录技术 

分 类 号：TF18[冶金工程—冶金物理化学]