Repetitive sequence based polymerase chain reaction to differentiate close bacteria strains in acidic sites  

作　　者：谢明 尹华群 刘毅 刘杰 刘学端 

机构地区：[1]Key Laboratory of Biometallurgy of Ministry of Education,School of Minerals Processing and Bioengineering,Central South University

出　　处：《中国有色金属学会会刊：英文版》2008年第6期1392-1397,共6页Transactions of Nonferrous Metals Society of China

基　　金：Projects(50621063,30428014)supported by the National Natural Science Foundation of China;Project(2004CB619201)supported by the National Basic Research Program of China

摘　　要：为了学习细菌的差异，紧张最新在中国从几酸矿排水( AMD )孤立地点，重复顺序基于的聚合酶链反应( rep-PCR )，为密切相关的细菌的差异分析的一种很好确定的技术拉紧，在细菌 Leptospirillum ferriphilium 的 30 紧张上被进行，细菌 Acidithiobacillus ferrooxidans 的 8 紧张，以及 Acidithiobacillus ferrooxidans 类型拉紧 ATCC (美国类型文化收集) 23270 。结果显示出那，用 ERIC 和盒子教材集合， rep-PCR 生产了高度差别对待的 banding 模式。基于 ERIC-PCR， banding 打的种系发生的分析被做，结果显示 rep-PCR 能在学习细菌的差异被用作一种快速、高度差别对待的屏蔽技术，特别在区分在在 AMD 的一种以内的细菌。To study the diversity of bacteria strains newly isolated from several acid mine drainage（AMD） sites in China, repetitive sequence based polymerase chain reaction （rep-PCR）, a well established technology for diversity analysis of closely related bacteria strains, was conducted on 30 strains of bacteria Leptospirillum ferriphilium, 8 strains of bacteria Acidithiobacillus ferrooxidans, as well as the Acidithiobacillusferrooxidans type strain ATCC （American Type Culture Collection） 23270. The results showed that, using ERIC and BOX primer sets, rep-PCR produced highly discriminatory banding patterns. Phylogenetic analysis based on ERIC-PCR banding types was made and the results indicated that rep-PCR could be used as a rapid and highly discriminatory screening technique in studying bacterial diversity, especially in differentiating bacteria within one species in AMD.

关 键 词：氧化亚铁 多样性 聚合酶链反作用 生物技术 

分 类 号：TF18[冶金工程—冶金物理化学]