异氟醚和地尔硫卓联合作用对人精子运动功能的影响  

Effects of Isoflurane Combined with Diltiazem on Human Sperm Motility

作　　者：许波[1] 汪小海[1] 王丽君[1] 孙慧谨[2] 

机构地区：[1]南京大学医学院附属鼓楼医院麻醉科,江苏南京210008 [2]南京医科大学附属南京市妇幼保健院检验科,江苏南京210004

出　　处：《中华男科学杂志》2008年第12期1109-1111,共3页National Journal of Andrology

摘　　要：目的：观察异氟醚和地尔硫卓联合应用在体外对人精子运动功能的影响并探讨其机制。方法：选取10例活力正常的精液标本，每例精液均一式9份，1份作为对照，其余8份分别经过不同浓度地尔硫卓单独或联合应用4．2％异氟醚处理1h，采用计算机辅助精液分析系统观察精子运动功能的变化情况。结果：①与对照组相比，0．01～1g／L地尔硫卓均可显著降低精子活力，并呈浓度依赖性降低，当地尔硫卓浓度升高至1g／L，精子活力显著降低。②将不同浓度的地尔硫卓和4．2％异氟醚联合应用后与同浓度下地尔硫卓组对比，0．01g／，L地尔硫卓与异氟醚联合用药时精子的活力显著升高，0．04g／L地尔硫卓与异氟醚联合用药时精子的活力无显著差别，0．2g／L地尔硫卓与异氟醚联合用药时精子的活力显著降低，1g／L地尔硫卓联合用药时精子的活力无显著差别。结论：异氟醚兴奋精子运动的作用可能与精子的钙离子通道有关，当此兴奋作用被地尔硫卓完全阻断时，异氟醚对精子的运动则表现出抑制作用。Objective： To observe the effects of isoflurane combined with dihiazem on human sperm motility in vitro and to investigate its possible mechanism. Methods ： Ten normal semen samples were collected, each divided into 9 groups, one as the control and the others treated in vitro with different concentrations of dihiazem or diltiazem ＋4.2% isoflurane for 1 hour. Sperm motility was observed with the computer-assisted sperm analyzer. Results： Compared with＇the control, dihiazem significantly decreased sperm motility at the concentrations of 0.01 g/L, 0.04 g/L, 0.2 g/L and 1 g/L in a dose-dependent manner, and reduced it to appreximately 0% at 1 g/L. When combined with 4.2% isoflurane, diltiazem obviously increased sperm motility at 0.01 g/L, markedly decreased it at 0.2 g/L, and effected no significant difference at 0.04 g,/L and 1 g/L as compared with the corresponding concentrations of diltiazem alone. Conclusion ： The stimulating effect of isoflurane on sperm motility may be associated with the calcium ion channel in sperm. When completely blocked by dihiazem, this effect may turn into an inhibition of sperm motility.

关 键 词：人 体外 异氟醚 地尔硫卓 精子活力 

分 类 号：R96[医药卫生—药理学] R614[医药卫生—药学]