人参皂甙Rg3对胰岛素样生长因子-Ⅰ基因敲除鼠乳腺癌组织血管生成相关基因的影响  

The Influence of Ginsenoside Rg3 on the Angiogenesis Relating Genes in Breast Carcinoma Tissue of IGF-Ⅰ-deficient Mice

作　　者：任玉萍[1] 叶子荣[1] 唐泓波[1] 张军[1] 高峰[1] 吴毅平[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院整形外科,武汉430030

出　　处：《华中科技大学学报（医学版）》2008年第6期724-727,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目(No.30271280)

摘　　要：目的探讨低血清胰岛素样生长因子-Ⅰ(IGF-Ⅰ)水平与正常水平的小鼠乳腺癌模型中,应用人参皂甙(GS)Rg3后IGF家族及血管生长因子相关基因的变化。方法7,12-二甲基苯蒽(DMBA)诱导肝脏特异性IGF-Ⅰ基因敲除(LID)鼠及对照鼠建立原发性乳腺癌模型,应用GSRg3进行干预治疗,利用基因芯片技术检测小鼠乳腺肿瘤及正常组织中相关基因的差异表达。结果①LID鼠组小鼠的肿瘤发生时间、生长速度及大小均低于对照组;②IGF家族及血管生长相关基因的变化:LID鼠组肿瘤组织中IGF-Ⅰ、IGFBP-4、IGFBP-7、FGF-1、FIGF、Ang-1、HGF、TGF-β1基因较对照组上调,IGF-Ⅱ、IGF-ⅡR、IGFBP-2、IGFBP-3、IGFBP-5、FLT-1、Ang-2、FGFR-2、PDGFa、PDGFb、THBS-1、Col18a1基因较对照组下调;应用GSRg3组IGF-ⅠR、IGF-ⅡR、IGFBP-2、IGFBP-3、IGFBP-5、VEGFa、Ang-2、EGF、EGFR、PDGFa、FG-FR-2、THBS-1、Col18a1基因较对照组上调,而IGF-Ⅰ、IGFBP-4、IGFBP-7、TEK、TGF-β1基因较对照组下调。结论IGF-Ⅰ促进小鼠乳腺癌的发生发展,且与血管生长密切相关。GSRg3可能通过IGF-Ⅰ及其结合蛋白、TEK、TGF-β1、THBS-1及Col18a1发挥抗肿瘤作用。Objective To determine the changes of insulin-like growth factor （IGF） genes and angiogenesis relating genes in breast carcinoma of mouse model of lower and normal serum levels of IGF-Ⅰ after treatment with Ginsenoside Rg3. Methods The breast carcinoma models with liver-specific IGF-Ⅰ -deficient （LID） mice in which serum IGF-Ⅰ level was 25% of that in control mice and control mice were established by feeding DMBA and treated with the angiogenesis inhibitor Ginsenoside Rg3. The genes in tumor tissues and normal tissues were detected by using gene chips. Results The tumorigenesis time, growth rate and the size of tumor in LID groups were decreased as compared with control groups. As compared with control group, the expression of IGF-Ⅰ, IGFBP-4, IGFBP-7, FGF-Ⅰ, FIGF, Ang 1 and TGF-β1 genes was increased and that of IGF-Ⅰ, IGF-ⅡR, IGFBP-2, IG-FBP-3, IGFBP-5, FLT-1, Ang-2, FGFR 2, PI^Fa, PDGFb, THBS-1 and Col18al genes decreased. After GSRg3 treatment the expression of IGF-ⅠR, IGF-ⅡR, IGFBP-2, IGFBP-3, IGFBP-5, VEGFa, Ang2, EGF, EGFR, PIX;Fa, FGFR-2, THBS-1, Co118al genes was increased and that of IGF-Ⅰ, IGFBP 4, IGFBP-7, TEK, TGF-β1 genes decreased in LID mice. Conclusion IGF-Ⅰ might be involved in progression of mouse breast cancer and be associated with the growth of vessels. Ginsenoside Rg3 may play an antitumor role by IGF-Ⅰ and its binding proteins, TEK, TGF-β1, THBS1 and Col18al.

关 键 词：胰岛素样生长因子-Ⅰ 人参皂甙RG3 基因芯片 肿瘤 

分 类 号：R349.7[医药卫生—基础医学]