受体血诱导大鼠肝移植免疫耐受的机制探讨  

Mechanism of hepatic allograft tolerance by pretransplant intraportal administration of recipient blood to the donor

作　　者：王凤山[1] 张佳林[1] 杨蕾[1] 程颖[1] 邵自功[1] 刘永锋[1] 

机构地区：[1]中国医科大学附属第一医院肝胆外科,器官移植科,沈阳110001

出　　处：《中华临床医师杂志（电子版）》2008年第12期41-44,共4页Chinese Journal of Clinicians(Electronic Edition)

基　　金：辽宁省教育厅高等学校科学研究项目(No.05L496);教育部留学回国人员科研启动基金资助项目(教外司留[2005]383号)

摘　　要：目的将受体血预先经门静脉输入供体,探讨肝移植后免疫排斥反应的变化。方法将LEW大鼠和ACI大鼠作为受体和供体。移植前7d将受体血经门静脉输入供体,移植后检测移植肝内供体源性树突状细胞,检测移植肝组织内IFN-γ mRNA的表达,检测移植肝内浸润细胞的凋亡。结果移植前7d受体血经门静脉输入供体组的生存时间显著延长,为(33.7±2.6)d,无处置组为(10.1±0.7)d。移植肝组织内IFN-γ mRNA有明显表达,移植肝内检测到大量供体源性树突状细胞。移植肝的浸润淋巴细胞凋亡显著多于无处置组。结论移植肝内浸润淋巴细胞凋亡抑制了肝移植排斥反应。Objective In the present study,we investigated the effect of the pretransplant intraportal administration of recipient blood to donor on rat hepatic allograft tolerance. Methods Male LEW （RT11） and ACI （RT1a） rats were used as liver transplant recipients and donors, respectively. Experimental groups were divided into four groups as follows：group Ⅰ, no treatment;group Ⅱ, donors pre-treated by the intravenous administration of LEW recipient blood 7 days before transplantation ; group Ⅲ, donors were pre-treated by the intraportal administration of third party BN blood 7 days before transplantation ; and group Ⅳ, donors were pre-treated by the intraportal administration of LEW recipient blood 7 days before transplantation. Serum levels of interferon （IFN）-γ were determined after transplantation. The number of dendritic cell （ CD62 ） of donor origin was measured with a monoclonal antibody against the rat RT1Ba class Ⅱ （1a） major histocompatibility complex （MHC）, OX76. Assessment of apoptotic hepatic infiltrates was determined. Results Animals in group Ⅰ survived a mean of 10. 1 ± 0. 7 days. The survival rate in group Ⅳ was prolonged significantly to 33.7 ± 2. 6 days. Serum concentrations of IFN-γ were increased significantly in group Ⅳ, as compared with group Ⅰ. The ratio of OX76 ^＋ CD62^＋ cells to CD62^ ＋ cells was greater in group Ⅳ, as compared group Ⅰ. Apoptotie hepatic infiltrates were greater in group Ⅳ treated by the intraportal administration of recipient blood, as compared with group Ⅰ. Conclusions Pretransplant intraportal administration of recipient LEW blood to ACI donor rats prolonged the survival of hepatic allograft. This may be associated with apoptosis of graft-infiltrating cells.

关 键 词：肝移植 免疫耐受 细胞凋亡 大鼠 

分 类 号：R657.3[医药卫生—外科学] R392[医药卫生—临床医学]