热化疗对K562细胞体外增敏作用的实验研究  

Thermotherapy enhances the sensitivity of K562 to chemotherapy in vitro:An experimental study

作　　者：魏红梅[1] 张立成[1] 张蕾[1] 宋毓敏[1] 郭坤元[2] 

机构地区：[1]88医院肿瘤科,山东泰安271000 [2]广州市南方医科大学珠江医院血液科,广东广州510282

出　　处：《实用医药杂志》2008年第12期1497-1499,1502,共4页Practical Journal of Medicine & Pharmacy

摘　　要：目的观察热疗联合阿霉素对人慢性白血病细胞株K562细胞内的药物浓度变化及凋亡的影响。方法MTT法确定阿霉素的工作浓度,以该浓度进行化疗或与热疗的联合,选择温度40、41、42℃,体外作用于K562细胞。作用前及作用48h采用台盼蓝拒染法检测肿瘤细胞的存活率;MTT法检测肿瘤细胞增殖的抑制作用;流式细胞仪检测肿瘤细胞的凋亡及细胞内药物浓度的变化。观察热疗联合阿霉素的抗肿瘤效果。结果作用48h IC50的药物浓度作为实验的工作浓度。热化疗组对K562细胞有明显的抑制作用(P<0.01),随着温度的增高而增强;热化疗组细胞内的药物浓度明显增加(P<0.01)。结论热疗联合阿霉素能增加K562细胞内的药物浓度,提高肿瘤细胞的凋亡率。Objective To observe the effect of thermotherapy on the intraeellular adriamycin concentration and apoptosis of K562 cells in vitro. Methods The working concentration of adriamycin against K562 was determined by MTT assay. K562 cells were subjected to thermotherapy （at 40℃, 41℃, 42℃） and chemotherapy with adriamycin alone or in conjunction, and the cell survival rates were determined at 48h after the treatment. The cell growth inhibition effect of the treatment was evaluated with MTT assay, and the apoptosis rates of K562 and alteration of intracellular adriamycin eoneentration were determined by flow cytometrie analyses. Results The IC50 of adriamycin was defined as its working concentration in the experiment. The thermotherapy at 40,41 and 42℃ for 60 rain in conjunction with chemotherapy significantly inhibited the growth of K562 cells （P〈0.01）. The results of flow cytometric analyses showed that thermotherapy and adriamycin chemotherapy, used either alone or in conjunction, obviously increased the apoptosis rates of K562 cells（P〈0.01） and thermotherapy remarkably increased the intracellular concentration of adriamycin. Conclusion Adriamycin chemotherapy combined thermotherapy for 60min can increase the intracellular concentration of adriamycin and the apoptosis rates of K562 cells.

关 键 词：热化疗 阿霉素 K562 增敏作用 细胞凋亡 

分 类 号：R453[医药卫生—治疗学] R454[医药卫生—临床医学]