大麻叶提取物及其活性成分齐墩果烯和齐墩果酸的抗菌活性研究  

Study on the Bacteriostatic Action to Extraction of Cannabis＇ Leaves and Bacteriostatic Component Oleanolic Alkene and Oleanolic Acid

作　　者：崔广东[1] 史高峰[1] 陈学福[1] 王国英[1] 吕玲玉[1] 吕秋楠[1] 

机构地区：[1]兰州理工大学石油化工学院,兰州730050

出　　处：《天然产物研究与开发》2008年第B05期48-51,共4页Natural Product Research and Development

基　　金：兰州市政府攻关项目;兰州市七里河区政府攻关项目;兰州理工大学博士基金

摘　　要：本文研究了低毒大麻叶不同极性浸膏的抑菌作用并对其活性抑菌物质进行跟踪，采用乙醇作溶剂对大麻叶的药用成分进行了粗提取，再用石油醚、乙酸乙酯、正丁醇溶剂进行萃取，并且对不同极性的浸膏进行抑菌实验的研究，对抑菌效果明显的浸膏进行化学物质的分离，采用UV、IR、NMR等波谱方法进行结构鉴定。对分离的化学物质进行抑菌实验的研究。结果表明，大麻叶的石油醚浸膏对金黄色葡萄球菌、绿脓杆菌、大肠杆菌和白色念珠菌有明显（P〈0．001）的抑制作用，同时低毒大麻叶石油醚浸膏中化学物质齐墩果烯和齐墩果酸（P〈0．001）的抑制作用更加明显。The bacteriostatic action of cannabis＇ leaves extraction were studied in this paper and we tracked cannabis＇ leaves bacteriostatic chemical component. First the pharmic components were extracted with alcohols ,then with petroleum ether,ethyl acetate and n-butanol respectively In this paper,the bacteriostatic actions of different extracts were studied, and the chemical components were sepovrated and identified by UV, IR and NMR methods. The bacteriostatic action of cannabis＇ leaves petroleum ether extract to Staphylococcus aureus,E, coliform,P, aeruginosa and Candida albicaus were obvious （ P 〈 0. 001 ）. The bacteriostatic actions of oleanolic alkene and oleanolic acid from petroleum ether extract were even more obvious （P 〈0. 001 ）.

关 键 词：石油醚浸膏 齐墩果烯 齐墩果酸 金黄色葡萄球菌 白色念珠菌 抑菌作用 

分 类 号：R282.71[医药卫生—中药学] S563.4[医药卫生—中医学]