麻花艽与小秦艽多糖含量的测定及其与生态环境的关系  

Determination of Polysaccharide in Gentiana straminea ＆ G. dahurica and Their Relationship with Ecological Environments

作　　者：孙菁[1] 李法强[2] 王延花[1,3] 诸葛芹[2] 陈桂琛[1] 

机构地区：[1]中国科学院西北高原生物研究所青海省青藏高原特色生物资源研究重点实验室,西宁810001 [2]中国科学院青海盐湖研究所,西宁810008 [3]中国科学院研究生院,北京100049

出　　处：《天然产物研究与开发》2008年第B05期55-58,110,共5页Natural Product Research and Development

基　　金：国家中西部专项基金项目（2001BA901A47）

摘　　要：采用紫外分光光度法，利用苯酚．硫酸进行显色，系统测定了两种传统中藏药材麻花艽与小秦艽药用部位的多糖含量，比较分析了不同产地二者多糖含量的差异，并初步探讨了生态环境因子中海拔和经纬度对其多糖含量的影响。结果表明，麻花艽多糖含量范围为1．53％-4．51％，平均含量为3．15％；小秦艽多糖含量范围为1．61％～3．44％，平均含量为2．24％，略低于麻花艽，且两个物种间多糖含量差异达到极显著水平（P〈0．01）。生态环境因子与多糖含量的相关分析结果显示，采样点的纬度对两种药材多糖含量具有一定的负面影响，即纬度越高，多糖含量越低，提示在利用麻花艽与小秦艽多糖资源时，纬度是采样点应首要考虑的环境因子。Through UV spectrophotometry and phenol-sulfuric acid color, the determinations of polysaccharide in the medicinal parts of two traditional Tibetan medicines Gentiarm straminea Maxim. and G. dahurica Fischer were developed and performed. Comparative analysis of contents of polysaccharide in different sampling localities and effects of ecological environment on the contents of pelysaecharide were also discussed in this study. The results showed that content of polysaccharide in G. straminea ranged from 1.53% to 4.51% ,with an average content of 3.15% ;while content of polysaccharide in G. dahurica changed in the range of 1.61% -3.44%, with an average content of 2.24%, slighdy lower than G. straminea,and polysaceharide content in these two species showed a significant difference （P 〈 0.01 ）. In order to further understand the effects of ecological environmental factors on the contents of pelysaccharide, correlation coefficient matrix between contents of polysaccharide and ecological environments was analyzed. The results showed that the latitude in the sampling localities of the two medicines had negative relationship with polysaccharide content, that is, the higher the latitude, the lower the polysaccharide content, which suggested that the latitude was a primary and important environmental factor in sampling localities to use polysaccharide in G. straminea and G. dahurica as medicines.

关 键 词：麻花艽 小秦艽 多糖含量 生态环境 

分 类 号：R927.2[医药卫生—药学]