X-5大孔树脂静态吸附分离咸草总黄酮特性的研究  

Study on Static Adsorbing and Desorbing Total Flavone from Cyperus malaccensis Lam.var.brevifolius with X-5 Macroporous Resin

作　　者：陈宏铭[1] 曾亚梅[1] 方玉霖[1] 黄敏参[1] 李桂源[1] 刘剑秋[1] 

机构地区：[1]福建师范大学生命科学学院,福建福州350108

出　　处：《福建师范大学学报（自然科学版）》2009年第1期105-109,共5页Journal of Fujian Normal University：Natural Science Edition

基　　金：福建省自然科学基金资助项目(D0610006)

摘　　要：通过比较4种大孔树脂对咸草总黄酮的吸附和解吸效果,筛选出适宜的X-5树脂,考察了咸草总黄酮提取液初始质量浓度、pH值、树脂用量、振荡温度、转速等因素对X-5树脂静态吸附咸草总黄酮的影响,以及V(乙醇)∶m(树脂)和乙醇体积分数对其静态解吸效果的影响.结果表明:当初始质量浓度为1.00～2.51 mg·mL-1,树脂用量为4 g,在35℃、转速为110 r/min振荡条件下,X-5树脂对咸草总黄酮有良好的吸附性能,pH对吸附的影响不显著;采用体积分数为60%乙醇,V(乙醇)∶m(树脂)=25∶1,其解吸效果较好,解吸率为75.73%.The appropriate X-5 Macroporous Resin was selected by camparing the dynamic total flavone adsorbtion and desorption curve of four types of macroporous resin. Effects of the initial concentration and pH of extract of total flavone, amount of resin, temperature and rotation speed of mechanical shaker on static adsorption, and effects of the V （ethanol） ： m （resin） , ethanol concentration on static seperation were investigated. The resuits showed that under the condition of the initial concentration 1.00- 2. 51 mg · mL^-1, resin 4 g, 35 ℃, 110 r/min, the capacity of X-5 Resin adsorbing total flavone was favorable. pH made no difference to adsorption , the desorption effect was fine with 60% ethanol, V （ethanol） ： m （resin） = 25 ： 1 . The desorption ratio was 75.73%.

关 键 词：X-5树脂 成草 总黄酮 

分 类 号：R284.2[医药卫生—中药学]