水黄皮根总黄酮抗DSS诱导的小鼠溃疡性结肠炎的实验研究  

Effect of total flavonoids of Pongamia pinnata roots on ulcerative colitis induced by dextran sulphate sodium in mice

作　　者：李靖[1] 朱毅[1] 陈国彪[1] 陈恩斌 黄凌[1] 赵锦[1] 

机构地区：[1]海南省药品检验所,海南海口570216 [2]武警海南省边防总队医院病理科,海南海口570208

出　　处：《中成药》2009年第1期31-34,共4页Chinese Traditional Patent Medicine

基　　金：海南省自然科学基金资助项目(30530)

摘　　要：目的:观察不同剂量的水黄皮根总黄酮(PRF)对右旋葡聚糖硫酸钠(DSS)诱导小鼠产生的溃疡性结肠炎(UC)的干预作用,并初步探讨其作用机制。方法:采用自由饮用4%DSS水溶液制备UC小鼠模型,以柳氮磺胺吡啶(SASP)作对照,观察各组小鼠的临床症状及结肠黏膜损伤情况,同时检测结肠组织中T-AOC、MDA、NO及NOS的水平。结果:与模型组比较,PRF高、中剂量组可不同程度的改善UC小鼠的临床症状,减轻结肠黏膜损伤,降低结肠组织中MDA、NO、NOS的含量或活性和升高T-AOC水平(P<0.05,P<0.01),且PRF高剂量组作用与SASP相似(P>0.05)。结论:PRF对DSS诱导的UC小鼠作用显著,其机制可能与减少自由基生成、抑制脂质过氧化反应和增强抗氧化能力作用有关。AIM： To study the effect of total flavonoids of Pongamia pinnata roots （PRF） on ulcerative colitis （UC） induced by dextran sulphate sodium （DSS） in mice and the possible mechanism. METHODS： Balb/c mice were fed with 4% DSS solution for 7 d to induce ulcerative colitis and treated with sulfasalazine （SASP） as positive control. The clinical symptom and the lesion of colonic mucosa were observed and the levels of T-AOC, MDA, NO and the activity of NOS in colonic tissue were tested. RESULTS ： Compared with the model control, the clinical symptom and the lesion of colonic mucosa in treatment group were remarkably improved after administration of PRF in large or medium dose. In the two groups, the decrease in the levels of MDA, NO and the activity of NOS, and the increase in the levels of F-AOC could be found. （P 〈0.05, P 〈0.01 ）. There was no significant difference between the large dosage PRF treated group and the SASP group （ P 〉 0.05 ）. CONCLUSION ： The results indicate that significant effect of PRF on DSS-induced UC may attributae to reduction in free radical, depression of lipid peroxidation and enhancement of antioxidant capability.

关 键 词：水黄皮根总黄酮 DSS 溃疡性结肠炎 T—AOC MDA NO NOS 

分 类 号：R285.6[医药卫生—中药学]