AB-8大孔吸附树脂纯化复方脑脉通有效部位的工艺研究  

Separation and purification of Naomaitong Granules by AB-8 macroporous absorption resin

作　　者：王淑美[1] 李淑芳[2] 梁生旺[1] 高淑娟[2] 李建生[2] 

机构地区：[1]广东药学院,广东广州510006 [2]河南中医学院,河南郑州450008

出　　处：《中成药》2009年第1期47-50,共4页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金资助项目(30371812)

摘　　要：目的:确定大孔吸附树脂纯化脑脉通(大黄、川芎、葛根等)有效部位的最佳工艺。方法:采用AB-8型树脂纯化脑脉通,以分光光度法测定洗脱物中总蒽醌、总皂苷和总生物碱的含量,HPLC法测定葛根素的含量,并以此为指标综合评价,优化脑脉通纯化工艺条件。结果:脑脉通药液的上样浓度为120 mg/mL(生药量),树脂柱的径高比为1∶10,最大的吸附量与树脂体积比为1∶6,水洗脱体积为2B倍,洗脱剂为50%乙醇,用量为8B倍树脂体积,洗脱流速2 mL/min。结论:有效部位纯度达63%,AB-8大孔吸附树脂对脑脉通的纯化工艺可行。AIM ： To study the purification of Naomaitong Granules （ Radix et Rhizoma rhei, Rhizoma chuanxiong, Radix Puerariae lobatae, etc） by macroporous absorption resin. METHODS： Naomaitong Granule was purificated by macroporous absorption resin AB-8. UV spectrothotometry was used to determine the contents of total anthraquinones, total ginsenosides,total alkaloids, and the content of puerarin was determined by HPLC. The technic of purification was optimized according to the content above. RESULTS： The optimized technological conditions consisted of eoncentranon of original sample 120 mg/mL, the diameter and height was in proportion of 1： 10, the ratio of maximum adsorption to resin volume was 1 ： 6, water elution was 2B multiple of resin volume, 8B multiple of resin volume 50% alcohol was the elution. CONCLUSION： AB-8 macroporous absorption resin can be used to purify Naomaitong Granules.

关 键 词：脑脉通 AB-8大孔吸附树脂 纯化工艺 

分 类 号：R284.2[医药卫生—中药学]