多波长RP-HPLC法测定抗感宁合剂中绿原酸、葛根素和芍药苷  

Simultaneous determination of chlorogenic acid,puerarin and paeoniflorin in Kangganning Mixture by multiple wavelength RP-HPLC

作　　者：靳淑敏[1] 杨维[2] 王伟华[1] 刘伟娜[2] 刘曼[2] 张兰桐[2] 王巧[2] 

机构地区：[1]石家庄市第一医院药剂科,河北石家庄050011 [2]河北医科大学药学院,河北石家庄050017

出　　处：《中成药》2009年第1期75-78,共4页Chinese Traditional Patent Medicine

基　　金：石家庄市科委课题项目(2004146853)

摘　　要：目的：建立同时测定抗感宁合剂（金银花，葛根，赤芍，连翘，等）中绿原酸、葛根素和芍药苷的分析方法。方法：采用多波长RP—HPLC法，色谱柱为Agilent Zorbax SB C18柱（250mm×4．6mm，5μm），乙腈-0．1％磷酸（11：89）为流动相，绿原酸、葛根素和芍药苷的检测波长分别为324nm，254nm和230nm，流速：1．0mL／min，柱温：30℃。结果：绿原酸、葛根素和芍药苷分别在0．179～2．864μg，0．07155—1．1448μg和0．3725～5．96μg范围内，其进样质量与峰面积呈良好的线性关系，平均回收率分别为100．7％，103．3％，102．6％，RSD分别为2．2％，2．4％，1．9％。结论：本法简便、准确、重现性好，可用于抗感宁合剂中绿原酸、葛根素和芍药苷的同时测定。AIM： To establish a method for simultaneously determining chlorogenic acid, puerarin and paeoniflorin in Kangganning Mixture （ Flos Lonicerae japonicae, Radix Puerariae Lobatae, Radix Paeoniale rubra, Fructus Forsythiae, etc. ）. METHODS： A multiple wavelength HPLC method was devoloped. The analysis was performed on an Agilent Zorbax SB C18 column （250 mm × 4.6 mm, 5 μm） with acetonitrile-0.1% H3PO4 （11：89） as the mobile phase. The detection wavelengthes were monitored at 324 nm, 254 nm and 230 nm for chlorogenic acid, puerarin and paeoniflorin, respectively. The flow rate was 1.0 mL/min and the column temperature was at 30 ℃. RESULTS ： The calibration curves of chlorogenic acid, puerarin and paeoniflorin showed good linearity at the ranges of 0. 179-2. 864 μg, 0.071 55-1. 144 8 μg and 0. 372 5-5.96 μg, respectively. The average recoveries were 100.7%, 103.3%, 102.6% with RSD of 2.2%, 2.4%, 1.9%, respectively. CONCLUSION： The method is simple, accurate and reproducible for determining chlorogenic acid, puerarin and paeoniflorin in Kangganning Mixture.

关 键 词：RP—HPLC 抗感宁合剂 绿原酸 葛根素 芍药苷 

分 类 号：R927.2[医药卫生—药学]