HPV16/18型表达和细胞病理学检查与宫颈癌及癌前病变的相关性研究  

A Study into Correlation of HPV16/18 Expression with Cytopathology Examination and Cervical Cancer and Precancerous Lesions

作　　者：张培影[1] 徐侠[1] 杨海燕[1] 杨静[1] 王旭波[1] 

机构地区：[1]南京中医药大学附属医院徐州市中医院,江苏徐州221003

出　　处：《南京中医药大学学报》2009年第1期27-30,81,共5页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：江苏省中医药局基金资助项目(HZ07099)

摘　　要：目的探讨细胞病理学和原位杂交技术(ISH)检测宫颈组织高危型HPV(16/18型)感染与宫颈癌及癌前病变发生的相关性。方法运用细胞病理学和原位杂交技术检测218例宫颈组织HPV16/18型的表达水平及病变情况,判定HPV16/18型感染对宫颈癌及癌前病变的筛查价值。结果218例低度鳞状上皮内瘤变(LSIL)以上患者HPV16/18检出率分别为:鳞状上皮轻度非典型增生(CIN)Ⅰ16.67%(15/90例)、鳞状上皮中度非典型增生(CIN)Ⅱ48.43%(31/64例)、鳞状上皮重度非典型增生(CIN)Ⅲ91.11%(41/45例),鳞状细胞癌(SCC)84.21%(16/19例)。CINⅢ和SCC组与CINⅠ、Ⅱ组间比较有非常显著性差异(P<0.01);CINⅢ与SCC组间比较差异无显著性(P>0.01)。结论原位杂交技术检测HPV16/18型对诊断宫颈癌及癌前病变具有敏感度、特异度高的特点;若原位杂交技术与细胞病理学诊断协同检测,可更早发现宫颈癌和癌前病变,并对宫颈病变干预和治疗具有重要的指导意义。OBJECTIVE To explore into the correlation between high-risk HPV （16/18 type） infection detected by in situ hybridization and cervical cancer and precancerous lesions. METHOD High risk HPV-DNA detection was given to 218 female cases by eytopathology and in situ hybridization （ISH）. The value of HPV16/18 type infection for cervical cancer and precancerous lesions was evaluated.RESULT The positive rates of HPV-DNA were 16.67% in CINI （ 15/90）, 48.43% in CINII （31/64）, 91.11% in CINIII （41/45）, 84.21% in SCC （16/19）. There was significant differences between the groups of CIN III, SCC and CIN Ⅰ＆Ⅱ （P 〈 0.01 ）. No significant differences were found in the groups of CIN Ill and SCC （ P 〉 0.01 ）. CONCLUSION In situ hybridization detection of HPV （16/18） is accurate and specific in detecting cervical cancer and precancerous lesions. The combined use of in situ hybridization with cytopathology ensures early detection of HPV, with great clinical value in improving cervical cancer and precancerous lesions.

关 键 词：宫颈癌 人乳头瘤病毒(HPV) 原位杂交(ISH) 细胞病理学 

分 类 号：R273.733[医药卫生—中西医结合]