益肾活血胶囊对高同型半胱氨酸血症兔平滑肌细胞增殖及MMP-9基因表达的影响  

Effect of Yishenhuoxue Capsule on Vascular Smooth Muscle Cell Proliferation and Matrix MMP-9 Gene Expression in Hyperhomocysteinemic Rabbits

作　　者：刘粉叶[1] 张继东[1] 胡连海[1] 来丽萍[1] 

机构地区：[1]山东大学齐鲁医院,山东济南250012

出　　处：《南京中医药大学学报》2009年第1期35-37,45,82,共5页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学基金(30572452)

摘　　要：目的探讨益肾活血胶囊对高同型半胱氨酸(Hcy)血症兔平滑肌细胞增殖及基质金属蛋白酶-9(MMP-9)基因表达的影响。方法40只新西兰兔,随机平分为正常组、模型组、益肾活血胶囊小剂量组、益肾活血胶囊大剂量组及西药(叶酸、维生素B6、维生素B12)组,除正常组外,其他组建立高Hcy血症及动脉粥样硬化(AS)动物模型,然后分别给予相应的药物治疗。高效液相色谱技术检测血浆Hcy浓度,HE染色观察血管形态学变化,透射电镜观察血管中膜平滑肌细胞超微结构,实时定量PCR检测动脉壁MMP-9基因表达。结果益肾活血胶囊和叶酸等维生素均可明显降低血浆Hcy水平,减轻血管内膜增度,抑制平滑肌细胞增生,下调腹主动脉MMP-9基因表达。益肾活血胶囊大剂量组疗效明显优于小剂量组和西药组。结论益肾活血胶囊可明显降低血浆Hcy水平,抑制Hcy诱导的动脉粥样硬化形成,其机制可能与抑制平滑肌细胞增殖,下调MMP-9基因表达有关。OBJECTIVE To explore into the effect of Yishenhuoxue Capsule on vascular smooth muscle cell proliferation and matrix metalloproteinase-9 （MMP-9） gene Expression in hyperhomocysteinemic rabbits.METHOD Forty male New Zealand rabbits were di- vided into five groups： control, model, low dose YC, high dose YC and western medicine （folic acid, Vitamin B6 and Vitamin B12）. A- part from the control group, the rabbits in other groups underwent balloon catheter injury and high methionine diet to induce hyperhomo- cysteinemia and atherosclerosis, followed by treatment with different drugs. The results were observed and evaluated. RESULT Both YC and western medicine could decrease the plasma Hey concentration, attenuate the internal thickening, inhibit VSMCs proliferation, and reduce MMP- 9 expression. High YC dosage group showed better results than low YC dosage group. CONCLUSION YC decreas- es plasma Hcy level and subsequently reverses the aortic lesion induced by Hcy. The mechanism may be related to its suppressive effect on MMP - 9 expression.

关 键 词：益肾活血胶囊 同型半胱氨酸 动脉粥样硬化 平滑肌细胞 基质金属蛋白酶-9 

分 类 号：R285.5[医药卫生—中药学]