发光细菌法中用发光二极管修正色度干扰的研究  

Color Correction in Luminescent Bacteria Toxicity Test Utilizing Light Emitting Diode for Measuring Toxicity of Colored Water Samples

作　　者：瞿璟琰[1] 王广鹤[1] 卢湘岳[1] 

机构地区：[1]华东师范大学环境科学与技术系,上海200062

出　　处：《环境科学与技术》2009年第2期102-105,共4页Environmental Science & Technology

基　　金：国家高技术研究发展"863"计划专项基金(2003AA601020)

摘　　要：在用发光细菌检测水样毒性时,水样的色度会干扰测量结果。该研究采用作者制作的发光二极管(LED)装置对水样色度引起的附加抑光读数进行修正。首先,利用食用色素溶液观察同种水样的色度对发光细菌和LED发光抑制的差异;其次,用坐标旋转变换的方法来矫正这一差异,并用食用色素和苯酚模拟有毒有色的水样,来验证方法的准确性。最后,用该修正方法检测染料活性黑KN-B溶液的毒性,并用双层管设置阴性对照来检验修正方法的可靠性。结果三组模拟水样得到的水样毒性与实际毒性之间的误差均<5%,表明该方法有效。测得活性黑KN-B的EC50为213.9mg/L,与先前用双层管色度修正法检测得到的结果相近。该研究为剔除由色度引起的附加抑光读数,准确反映水样本身毒性,提供了一种可行且较可靠的方法。When colored water sample was measured with luminescent bacteria toxicity test, the color of sample will affect the detection for luminescence. A light emitting diode （LED） installation was used to correct the depression of luminescence which was Caused by sample color. There was difference between bacteria and LED luminescence by detecting edible colorant solution, then the coordinate rotation converting method was used to rectify the difference, which was authenticated using simulated water sample made by edible colorant and phenol. Finally, the toxicity of activity blank KN-B solution was detected by the method, and co-concentric double-deck test tubes were used to check the reliability of rectification. Results showed that the relative errors between sample toxicity and real toxicity were less than 5%, which indicated the method was effective. EC50 of KN-B was 213.9mg/L, which was similar to the result detected by double-deck test tubes. The method was found available to reject the luminescence depression caused by sample color.

关 键 词：发光细菌 有色水样 发光二极管 坐标旋转变换 

分 类 号：X830.2[环境科学与工程—环境工程]