水稻灌浆期叶片蛋白质差异表达及其作用机理分析  

Differential Expression and Function Analysis of Proteins in Flag Leaves of Rice during Grain-Filling Period

作　　者：李兆伟[1] 熊君[1,2] 齐晓辉[1,2] 王经源[1,2] 陈鸿飞[1] 张志兴[1] 黄锦文[1] 梁义元[1] 林文雄[1,2] 

机构地区：[1]福建农林大学农业生态研究所 [2]福建农林大学生命科学学院,福建福州350002

出　　处：《作物学报》2009年第1期132-139,共8页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(30600385);福建省重大科技项目(2004NZ01-4);福建省自然科学基金项目(2008J0042)资助

摘　　要：采用双向电泳和质谱技术研究大穗型水稻"金恢809"灌浆期旗叶的蛋白质表达模式,共发现17个蛋白质发生差异表达,其中3个在灌浆前期至中期大量表达,9个在中后期大量表达,4个在后期大量表达,1个在灌浆前期和后期出现两次表达高峰。经MALDI-TOF/MS分析和数据库检索,鉴定出12个差异蛋白质,分别参与叶片的物质合成与降解,碳水化合物运输,植株抗氧化反应,激素代谢,以及细胞骨架的构建和组织成熟等生理反应。分析结果表明,核糖/半乳糖/甲基半乳糖苷运输ATP结合蛋白1在灌浆前中期参与物质向籽粒的运输;生长素响应蛋白IAA27通过调节ATPase活性影响叶片物质运输;N-乙酰谷氨酸半醛脱氢酶在灌浆末期参与叶片的多胺代谢,延缓叶片衰老;谷胱甘肽S-转移酶和Cu/Zn-超氧化物歧化酶在籽粒灌浆后期的植物解毒和防御活性氧伤害中起着重要作用。The experiment was conducted to study the protein expression pattern in flag leaves of rice cultivar Jinhui 809 with large panicles and high grain-setting percentage during grain-filling period using two-dimensional electrophoresis and mass spectrometry. The results showed that there were 17 protein spots with differential expression. Among them, three protein spots had the most expression amount from early to mid-stage, nine protein spots had the most expression amount from middle to late stages, four protein spots reached the most expression amount in late stage, and one protein spot had two top expression peaks in early and late stages of grain filling, respectively. MALDI-TOF/MS analysis and database searching identified 12 differential proteins involved in biosynthesis and degradation, carbohydrate transportation, anti-oxidation reaction, hormone metabolism, cytoskeleton construction and tissue maturation, respectively. Ribose/galactose/methyl galactoside importing ATP-binding protein 1 was participated in photosynthates transportation from leaf to grain in early and mid-stages. Auxin-responsive protein IAA27 influenced photosynthates transportation by regulating the activity of ATPase. N-acetyl-glutamate semialdehyde dehydrogenase delayed leaf senescence through regulating polyamine metabolism in late stage. In addition, glutathione S-transferase and superoxide dismutase played an important role in plant detoxification and fighting against oxygen species damage at late stage of grain filling. The current results provide the valuable evidences for us to reveal the metabolism mechanism at grain filling stage in super-rice.

关 键 词：水稻 灌浆 叶片 蛋白质组学 

分 类 号：S511[农业科学—作物学]