傅立叶变换红外光谱技术在茧质检测中的应用  

Study on the Quality of Silk Cocoons by FT-IR

作　　者：陈植成[1,2] 黄庶识[1] 刘军贤[2] 陶站华[1] 顾家栋 王一兵 

机构地区：[1]广西科学院生物物理实验室,南宁530007 [2]广西师范大学物理与电子工程学院,广西桂林541004 [3]广西壮族自治区蚕业技术推广总站,南宁530007

出　　处：《丝绸》2009年第1期31-33,共3页Journal of Silk

基　　金：广西科学院基本科研业务费资助项目(桂科院研0702);广西研究生教育创新计划项目(2007106020702M55)

摘　　要：利用红外光谱技术分析不同上蔟环境营茧的蚕茧,通过对蚕茧蛋白酰胺I带和酰胺Ⅱ带进行曲线拟合,分离出重叠的子峰。以β折叠构象子峰峰面积与无规卷曲子峰峰面积的比值N的大小来衡量蚕茧解舒率的优劣,N值越大解舒率越低。结果为高温潮湿上蔟环境蚕茧的β折叠构象含量最高N=0.773,通风上蔟环境蚕茧的β折叠构象含量次之N=0.580,正常上蔟环境蚕茧的β折叠构象含量最低N=0.489,因此通风和正常上蔟环境蚕茧的解舒率较高。Different silk cocoons that spun in moist, ventilated and controllable surroundings were analyzed by FT-IR. Through curve fitting, the unidentified component peaks of the Amide Ⅰ and Ⅱ bands were isolated in the spectra. The ratio N of square β-sheet peak to square unorder peak in the spectra was used to measure reelability percentage of cocoons. The results showed that the content of β-sheet conformation in 3 kinds of cocoons was distinguishing： the moist was the highest（N = 0.773）,the ventilated took second place （N= 0.580）, and the controllable was the minimum （N = 0.489）. Therefore, the reelability percentage of the cocoon spun in the moist surrounding was lower than that of the cocoons spun respectively in the controllable and ventilated circumstances.

关 键 词：蚕茧质量检测 FT—IR 上蔟环境 丝胶蛋白质 曲线拟合 二级构象 

分 类 号：TS143.214[轻工技术与工程—纺织材料与纺织品设计] S886.3[轻工技术与工程—纺织科学与工程]