RF-PECVD聚合类聚乙烯氧(PEO-like)薄膜及其对血小板吸附性研究  

Surface Adsorption of Platelet-Rich Plasma of Polyethylene Oxide Like Films

作　　者：胡文娟[1] 谢芬艳[1] 付亚波[1] 陈强[1] 翁静[2] 

机构地区：[1]北京印刷学院印刷包装材料与技术北京市重点实验室,北京102600 [2]首都医科大学基础医学部,北京100069

出　　处：《真空科学与技术学报》2009年第1期85-89,共5页Chinese Journal of Vacuum Science and Technology

基　　金：国家自然基金(No.1775017);北京市留学人员重点基金;北京人才强校拔尖人才计划(PHR(IHLB));印刷包装材料与技术北京市重点实验室开放课题(No.KF060201)资助

摘　　要：本文采用平板式电容耦合射频(RF,13.56MHz)等离子体源,以乙二醇二甲基醚(Ethylene Glycol Di Methyl Ether)为聚合单体,氩气为辅助气体,在连续与脉冲射频等离子体两种放电模式下合成类聚乙烯氧(PEO-like)功能聚合薄膜。实验研究了等离子体放电参数:等离子体放电功率、工作气压、放电模式(连续或脉冲)和聚合时间等对聚合物表面结构、功能团含量、表面成分性能以及和血小板吸附等影响。利用接触角测定仪(WCA)、傅里叶变换红外光谱(FTIR)、原子力显微镜(AFM)等手段对聚合薄膜的结构、成分和形貌进行细致的分析。同时本文还进行体外细胞培养法,研究了类PEO功能薄膜对富血血小板的吸附,通过倒置显微镜观察细胞黏附的数量和形态变化。得到的结论为:采用RF-PECVD可以在较小功率的连续等离子体放电模式,或较长脉冲间隔的脉冲放电模式下得到结构稳定的PEO生物功能薄膜,所制备的PEO生物功能薄膜具有良好的抗血小板吸附性能。The polyethylene oxide like（PEO-like） functional films were polymerized by plasma enhanced chemical deposition （PECVD） on p-type Si（100） substrates with ethylene glycol dimethyl ether（EGDME） as the precursor.-The continuous wave （CW） and pulsed plasmas were used. The influence of the fill polymerization conditions, such as plasma power, Ar flow rate, pressure, discharge （CW or pulsed） modes and substrate temperature, polymerization time on the protein adsorption of the functional films was studied. The microstruetures and chemical composition of the films were characterized with atomic force microscopy （AFM）, Fourier transform infrared spectroscopy （FTIR） and water contact angle （WCA） measurement. The results show that a judicious choice of polymerization conditions significantly affects the performance of the functional films. The fairly stable PEO-like films, which strongly adsorbs platelet-rich plasma（PRP）, can be polymerized by RF PECVD technique under the conditions of CW plasma with small power, or under the pulsed plasma with high duty cycle.

关 键 词：RF—PECVD PEO-like 血小板吸附 

分 类 号：TB34[一般工业技术—材料科学与工程] O646.9[理学—物理化学]