黄芩甙对重症急性胰腺炎TNF-α、IL-6及IL-10的影响  

Effects of baicalin on TNF-α,IL-6 and IL-10 in rats with severe acute pancreatitis

作　　者：李慧艳[1] 赵曙光[1] 赵保民[1] 王旭霞[1] 唐华[1] 冯国华[1] 王庆莉[1] 闻勤生[1] 

机构地区：[1]第四军医大学唐都医院消化内科,陕西西安710038

出　　处：《西南国防医药》2009年第1期26-29,共4页Medical Journal of National Defending Forces in Southwest China

摘　　要：目的：研究黄芩甙对重症急性胰腺炎（SAP）中组织TNF-α、IL-6、IL-10及TNF-α／IL-10动态变化的影响。方法：雄性SD大鼠45只，随机分为假手术组、模型组、干预组，每组各15只。假手术组仅行开腹翻动胰腺十二指肠后关腹，其余两组开腹后均行3.5％牛黄胆酸钠逆行胰胆管注射，制作SAP模型。造模后10min，干预组经股静脉给予5％黄芩甙，其余两组给予等量生理盐水。给药3、6、12h后测定血清淀粉酶、脂肪酶；观察胰腺组织病理改变；测定胰腺组织中TNF-α、IL-6、IL-10的含量。结果：（1）模型组血清淀粉酶、脂肪酶较假手术组显著增高（P〈0．01）；除3h脂肪酶外，干预组各时间点血清淀粉酶、脂肪酶较模型组显著降低（P〈0．01～0．05）；（2）模型组胰腺及周围有皂化斑的形成，胰腺水肿、出血、炎细胞浸润，胰腺组织结构破坏明显，有大片坏死区；干预组与模型组比较显著改善；（3）模型组胰腺组织中TNF-α、IL-6、IL-10水平较假手术组显著升高（P〈0．01），而干预组较模型组显著降低（P〈0．01～0．05）；（4）模型组TNF-α/IL-10随时间先升高，6h后逐渐减低；干预组较模型组降低早，3h已开始明显降低。结论：黄芩甙可能通过减少TNF-α和IL-6的产生和释放，下调TNF-α/IL-10发挥对胰腺组织的保护作用。Objective： To investigate the effects of baicalin on TNF-α, IL - 6, IL - 10 and the ratio of TNF-α./IL - 10 in rats with severe acute pancreatitis （SAP）. Methods：45 SD male rats were randomly divided into sham - operated group, model group and baicalin treated group and each had 15 rats. Rats in sham - operated group were only performed laparotomy and fliped intestines without any other treatment, however, those in other two groups were conversely injected 3.5% sodium taurocholate into pancreatic duct to induce the SAP models, and 10 rain later, 5% baicalin was given via thigh vein in baicalin treated group while equal dose of saline was injected into the other two groups. At 3, 6, 12 h after administration,the levels of serum amylase and serum lipase were determined, the pathological changes of pancreas were observed,and the content of TNF-α, IL - 6, IL - 10 in pancreas was detected, respectively. Results：Compared with sham - operatod group,the levels of serum amylase and serum lipase in model group increased significantly at different time points （ P 〈 0.01 ）. Compared with model group, the levels of serum amylase and serum lipasc in haicalin treated group decreased significantly （P 〈 0.01 ～0.05 ） except lipase level after 3 h. There was no obvious structural changes in rat pancreas in sham - operated group while the edema, bleeding and saponified spots in pancreas were found in model group. However, pathological changes in baicalin treated group were significantly improved compared with model group. The content of TNF-α, IL - 6, IL - 10 in pancreatic tissue in model group was significantly higher than those in other two group （ P 〈 0.01 - 0.05 ）. In model group, TNF-α/IL - 10 increased firstly with time passing and then decreased gradually after 6 h. In baicalin treated group, TNF-α/IL - 10 decreased earlier, but there was an obvious decrease after 3 h. Conclusion： Baicalin can inhibit the production and releasing of TNF-α and IL - 6 and reduce the ratio of TNF-α/IL - 10

关 键 词：急性胰腺炎 黄芩甙 TNF-Α IL-6 IL-10 

分 类 号：R285.5[医药卫生—中药学]