氯胺酮对体外循环诱导外周血单核细胞NF-κB及TNF-α表达的影响  

Effects of ketamine on expression of NF-κB and TNF-α in PBMCs induced by cardiopulmonary bypass

作　　者：魏晓红[1] 刘小燕[1] 李军[2] 杨小云[1] 刘宝玉[1] 刘合年[2] 

机构地区：[1]成都军区总医院胸心外科,四川成都610083 [2]成都军区总医院麻醉科,四川成都610083

出　　处：《西南国防医药》2009年第1期48-50,共3页Medical Journal of National Defending Forces in Southwest China

摘　　要：目的:观察氯胺酮对体外循环(CPB)诱导外周血单核细胞(PBMCs)中核因子-κB(NF-κB)和肿瘤坏死因子(TNF-α)表达的影响。方法:选择先天性室间隔缺损心脏病需行体外循环心脏直视手术患儿20例,随机双盲分为对照组和氯胺酮组,每组10例。氯胺酮组在CPB开始时立即给予1 mg/kg氯胺酮。采用Western blot和RT-PCR测定CPB前(T0)、CPB30 min(T1)、停CPB后1 h(T2)、停CPB后4 h(T3),PBMCs中NF-κB P65及TNF-αmRNA的表达。结果:两组PBMCsNF-κB P65和TNF-αmRNA表达水平在T2、T3时均有不同程度升高,但在T2、T3时氯胺酮组的表达水平显著低于对照组。结论:氯胺酮对CPB诱导的PBMCs NF-κB和TNF-α表达具有一定的抑制作用。Objective：To investigate the effects of ketamine on the expression of nuclear factor- κB （NF - κB） and tumor necrosis factor - α （ TNF - α） in peripheral blood mononclear cells （PBMCs） induced by cardiopulmonary bypass （CPB）. Methods： Twenty child patients who would undergo elective ventrieular septal defect correction were randomly divided into control group and ketamine group. The patients of ketamine group received 1mg/kg ketamine at beginning of CPB. At T0 （heft,re CPB）, T1 （CPB for 30 min）, T2 and T3 （ 1 h and 4 h after CPB）, the expression level of NF - κB P65 and TNF - α mRNA were detected by Western blot and RT - PCR in PBMCs. Results：The expression level of NF - κB P65 and TNF - α mRNA in PBMCs increased at T2 and T3 in both groups, but expression level in ketamine group were markedly down regulated compared with those in control gronp. Conclusion： Ketamine may inhibit the expression of NF - κB and TNF-α induced by CPB.

关 键 词：氯胺酮 体外循环 核因子-ΚB 肿瘤坏死因子-Α 

分 类 号：R654.1[医药卫生—外科学]