低能Ar^+注入对玉米花粉萌发及Ca^(2+)浓度分布的影响研究  

Effects of Low Energy Argon Ion Implantation on Maize Pollen Germination and the Cytosolic Ca^(2+) Distribution during Pollen Germination Process

作　　者：邓方[1] 程备久[2] 朱苏文[2] 

机构地区：[1]巢湖学院,安徽巢湖238000 [2]安徽农业大学生命科学院,安徽合肥230036

出　　处：《安徽农业科学》2008年第34期14963-14966,15095,共5页Journal of Anhui Agricultural Sciences

摘　　要：[目的]研究低能离子注入对花粉萌发及萌发过程中游离Ca2+的影响。[方法]利用能量为30 keV,剂量为0.78×1015~13×1015ion/cm2的Ar+注入玉米花粉粒,研究注入后玉米花粉的萌发情况和萌发过程中游离Ca2+的分布情况。[结果]当Ar+注入剂量为5.2×1015ions/cm2时,玉米花粉的萌发率有了明显的提高;而注入剂量超过7.8×1015ion/cm2以后,萌发率呈急剧下降趋势;同时利用低温孵育法在完整的玉米花粉粒中,载入酯化形式的Ca2+荧光探针fluo-3 AM后发现,花粉细胞中游离Ca2+浓度变化与花粉萌发活性变化相一致。[结论]低能离子注入后引起的花粉内Ca2+浓度的动态变化可能是花粉萌发变化的初始效应。[Objective] The purpose of this study is to investigate the effect of low energy ion implantation on maize pollen germination and cytosolic Ca2＋ distribution during pollen germination process.[Method] The Ar＋ with energy of 30 keV,dose of 0.78×1015～13×1015 ion/cm2,was implanted into maize pollen by bombardment,and the germination of pollen and cytosolic Ca2＋ distribution during pollen germination process of the Ar＋ implanted pollen were studied.[Result] When been bombarded with argon ions with dose of 5.2×1015 ion/cm2,the germination rate of maize pollen increased remarkably,while implantation of ions with dose exceeding 7.8×1015 ion/cm2 sharply decreased the germination rate of maize pollen.Meanwhile,tracing of esterified Ca2＋ fluorescent probe fluo-3 AM in intact pollen showed that variation of cytosolic Ca2＋ concentration was consistent with the change of pollen fertility.[Conclusion] The dynamics of cytosolic Ca2＋ concentration caused by low energy ion implantation may be concluded as the initial effects of pollen germination.

关 键 词：Ar+ 玉米 花粉萌发 CA2+ 浓度分布 

分 类 号：S513[农业科学—作物学]