胆石致胆管狭窄伴肝纤维化胶原蛋白的免疫组化观察  

IMMUNOHISTOCHEMICAL STUDY ON COLLAGENS OF HEPATOBILIARY DUCT STRICTURE DUE TO STONES WITH LIVER FIBROSIS

作　　者：夏先明[1,2,3] 张陪明[1,2,3] 刘广益[1,2,3] 雷正明[1,2,3] 肖永成[1,2,3] 

机构地区：[1]泸州医学院附院肝胆研究室 [2]四川省第五人民医院 [3]泸州医学院组织胚胎学教研室

出　　处：《中国普外基础与临床杂志》1998年第2期75-77,共3页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：对２１例择期手术定位取材所获得的５例左右肝管汇合部狭窄、７例Ⅰ级肝管狭窄、７例Ⅱ级肝管汇合部狭窄、１０例Ⅱ级肝管狭窄及１０例Ⅲ级肝管狭窄标本进行Ⅲ、Ⅳ型胶原蛋白的免疫组化观察及定量分析。结果：正常胆管壁、门脉区及肝窦的Ⅲ型胶原呈浅淡而不连续的阳性染色，而狭窄胆管壁、门脉区及肝窦的Ⅲ型胶原则呈连续粗线状阳性染色，与正常对照组比较，其差异非常显著（Ｐ＜０．０１）；在有胆管狭窄处的肝窦部，Ⅳ型胶原呈连续粗线状阳性染色。Immunohistochemical study on 39 specimens of hepatobilibary duct stricture due to stones were performed. Collagen types Ⅲ and Ⅳ were studied by quantitative analysis. The results showed that significant increase of type Ⅲ collagen was found in the stenotic bile duct wall, the portal area and liver sinusoid with fibrosis. Abnormal increasing of type Ⅳ collagen was found in the liver sinusoid of the stenotic bile duct.

关 键 词：胆管狭窄 肝纤维化 胶原蛋白 免疫组织化学 胆石 

分 类 号：R657.46[医药卫生—外科学] R575.5[医药卫生—临床医学]